{"id":"W2160998528","doi":"10.1101/pdb.prot072801","title":"Loading Fluorescent Ca<sup>2+</sup> Indicators into Living Cells","year":2013,"lang":"en","type":"article","venue":"Cold Spring Harbor Protocols","topic":"Protein Kinase Regulation and GTPase Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University","funders":"","keywords":"Fluorescence; Chemistry; Membrane; Pinocytosis; Carboxylic acid; Pipette; Microinjection; Hydrolysis; Biophysics; Intracellular; Cell; Biochemistry; Endocytosis; Cell biology; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0003412811,0.0002660741,0.0002125201,0.0001390845,0.0002140421,0.0001619143,0.0003374727,0.0002131923,0.000246465],"category_scores_gemma":[0.0001314631,0.0002715096,0.0001293354,0.0001927721,0.0000758005,0.00001807102,0.0002696139,0.0001821412,0.0002222223],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006857063,"about_ca_system_score_gemma":0.0001179745,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00008474342,"about_ca_topic_score_gemma":0.00000169207,"domain_scores_codex":[0.9983131,0.00009719966,0.0003654509,0.0005200313,0.0002501364,0.0004541391],"domain_scores_gemma":[0.9989198,0.00001937536,0.0001544751,0.0005574518,0.00009886699,0.0002500424],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001439596,0.00007880639,0.002213715,0.0001371839,0.00002982207,0.000004179542,0.00008299102,0.000698464,0.9950108,0.0003777329,0.0007181876,0.000633742],"study_design_scores_gemma":[0.0004132729,0.0001177659,0.001613633,0.0003494293,0.000009082158,6.480303e-7,0.00002709417,0.003037028,0.9346244,0.00001599822,0.05941462,0.0003770216],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9342241,0.0002819159,0.006355983,0.0001534729,0.00007717517,0.05841216,0.000006113974,0.0001385909,0.0003505367],"genre_scores_gemma":[0.9470757,0.000006523915,0.003620144,0.0002648276,0.0003412498,0.04847164,0.000002667884,0.00005778773,0.0001595057],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.06038637,"threshold_uncertainty_score":0.9999737,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00844166985679403,"score_gpt":0.2423057089647464,"score_spread":0.2338640391079523,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}