{"id":"W2160998528","doi":"10.1101/pdb.prot072801","title":"Loading Fluorescent Ca<sup>2+</sup> Indicators into Living Cells","year":2013,"lang":"en","type":"article","venue":"Cold Spring Harbor Protocols","topic":"Protein Kinase Regulation and GTPase Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University","funders":"","keywords":"Fluorescence; Chemistry; Membrane; Pinocytosis; Carboxylic acid; Pipette; Microinjection; Hydrolysis; Biophysics; Intracellular; Cell; Biochemistry; Endocytosis; Cell biology; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007012816,0.0006615449,0.0007917499,0.0003825092,0.0005094341,0.0008658799,0.000856404,0.0009224556,0.006583737],"category_scores_gemma":[0.0006159848,0.0003660247,0.0005674333,0.0005999154,0.0005595107,0.0009498558,0.0007722243,0.001140353,0.005424699],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005499696,"about_ca_system_score_gemma":0.0004543735,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004391854,"about_ca_topic_score_gemma":0.001055524,"domain_scores_codex":[0.9994015,0.00005769854,0.00008269263,0.0001417475,0.0002133608,0.000103045],"domain_scores_gemma":[0.9995489,0.000166908,0.00004425017,0.0001198214,0.00006903509,0.00005121697],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.00008049443,0.00002495882,0.00008308468,0.0001133452,0.000007027034,0.00007715212,0.00005406403,0.000097216,0.9912918,0.0006865439,0.001153536,0.006330773],"study_design_scores_gemma":[0.00002036356,0.00009379168,0.0003356274,0.00001187911,0.00001278887,0.00007669925,0.00001430079,0.0005981047,0.9801663,0.000179279,0.01848137,0.000009645481],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"empirical","genre_gemma":"protocol","genre_scores_codex":[0.570818,0.01041733,0.3246504,0.003379548,0.002886915,0.002887738,0.008927392,0.01006799,0.0659647],"genre_scores_gemma":[0.5280806,0.01153186,0.3849304,0.002118381,0.0005776444,0.004152714,0.01065139,0.002460493,0.05549654],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.006583737,"threshold_uncertainty_score":0.02202481,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00844166985679403,"score_gpt":0.2423057089647464,"score_spread":0.2338640391079523,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}