{"id":"W2161829824","doi":"10.1093/nar/gku651","title":"Design and characterization of a dual-mode promoter with activation and repression capability for tuning gene expression in yeast","year":2014,"lang":"en","type":"article","venue":"Nucleic Acids Research","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":39,"is_retracted":false,"has_abstract":true,"ca_institutions":"SGS (Canada); Impact; University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada; University of Toronto","keywords":"Psychological repression; Biology; Lac repressor; Synthetic biology; Repressor; Upstream activating sequence; Promoter; TATA box; lac operon; Regulation of gene expression; Corepressor; Computational biology; Gene; Gene expression; Genetics; Cell biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004211022,0.000304548,0.0003159371,0.0002480236,0.0001498979,0.0004218276,0.0004928718,0.0003128391,0.0003179511],"category_scores_gemma":[0.000346846,0.0002457146,0.0003613091,0.0002667389,0.0002267602,0.0002186415,0.0002475112,0.0005800544,0.0003308746],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003928418,"about_ca_system_score_gemma":0.0004083303,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001154715,"about_ca_topic_score_gemma":0.000843672,"domain_scores_codex":[0.9997759,0.00003068736,0.00003498569,0.00004913206,0.00007832175,0.00003087364],"domain_scores_gemma":[0.9997835,0.00003888661,0.00004278425,0.00003211597,0.00004707654,0.00005564971],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003588905,0.00002396225,0.0001697358,0.00002627948,0.000002385186,0.00002461265,0.00001769769,0.0003365986,0.9977495,0.0001230333,0.00001151415,0.001478727],"study_design_scores_gemma":[0.00001404238,0.0001146043,0.0005282405,0.000005256592,0.000009384104,0.00009866883,0.00001986047,0.001982041,0.9951074,0.00003207918,0.002080782,0.000007731724],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9074336,0.001019644,0.08892567,0.0001765204,0.0000333555,0.0002311273,0.0008877671,0.0003532461,0.0009390373],"genre_scores_gemma":[0.8801289,0.0008442216,0.1138981,0.00005370656,0.00001062241,0.0001709469,0.002278173,0.0002060967,0.00240926],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001154715,"threshold_uncertainty_score":0.002850235,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02678427990522865,"score_gpt":0.3166193549759825,"score_spread":0.2898350750707538,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}