{"id":"W2161829824","doi":"10.1093/nar/gku651","title":"Design and characterization of a dual-mode promoter with activation and repression capability for tuning gene expression in yeast","year":2014,"lang":"en","type":"article","venue":"Nucleic Acids Research","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":39,"is_retracted":false,"has_abstract":true,"ca_institutions":"SGS (Canada); Impact; University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada; University of Toronto","keywords":"Psychological repression; Biology; Lac repressor; Synthetic biology; Repressor; Upstream activating sequence; Promoter; TATA box; lac operon; Regulation of gene expression; Corepressor; Computational biology; Gene; Gene expression; Genetics; Cell biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006072528,0.00008619651,0.0001105511,0.00008002957,0.0001138313,0.00002556996,0.00005192856,0.0001134871,0.00000608364],"category_scores_gemma":[0.0001785838,0.00006752834,0.00001489905,0.00008646939,0.0001109518,0.00001719757,0.0001051074,0.00008709352,1.93553e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001159645,"about_ca_system_score_gemma":0.00003529536,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001606875,"about_ca_topic_score_gemma":0.000002554824,"domain_scores_codex":[0.9989172,0.00025909,0.0001453929,0.0003444847,0.0001604652,0.0001733453],"domain_scores_gemma":[0.9994528,0.00003875622,0.00006114777,0.0002244834,0.0001573616,0.00006540553],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006993737,0.0000656235,0.005658714,0.00008154444,0.000004959636,1.940381e-7,0.0001872732,0.00006616512,0.9889736,0.000008112923,0.0000311056,0.004223309],"study_design_scores_gemma":[0.0007422742,0.0007415353,0.02730905,0.0001342897,0.00000272704,0.000002891903,0.00003363632,0.005258808,0.9653302,0.0001739262,0.0001848606,0.00008577886],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9449592,0.00002211495,0.05437325,0.00004887958,0.00001276809,0.0005297278,0.000008744616,0.000005447913,0.00003990224],"genre_scores_gemma":[0.9965509,0.00004838524,0.003107526,0.00001455028,0.00005817546,0.000107581,0.00004923912,0.00001702287,0.00004664964],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.05159171,"threshold_uncertainty_score":0.2753726,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02678427990522865,"score_gpt":0.3166193549759825,"score_spread":0.2898350750707538,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}