{"id":"W2161902864","doi":"10.1128/jb.00141-10","title":"Membrane Topology and Identification of Critical Amino Acid Residues in the Wzx O-Antigen Translocase from <i>Escherichia coli</i> O157:H4","year":2010,"lang":"en","type":"article","venue":"Journal of Bacteriology","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":29,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"Canadian Institutes of Health Research","keywords":"Aspartic acid; Periplasmic space; Biology; Biochemistry; Membrane topology; Lysine; Amino acid; Cysteine; Threonine; Alanine; Peptide sequence; Serine; Escherichia coli; Transmembrane domain; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005629924,0.0001373064,0.0003357937,0.00008464339,0.00004065881,0.00001784721,0.0003640054,0.0005135523,0.00004044078],"category_scores_gemma":[0.0002719749,0.0001006543,0.00008253581,0.0000788029,0.0006391935,0.000007736314,0.00005913782,0.0003812362,0.000001142694],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000003491162,"about_ca_system_score_gemma":0.00007252576,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000563235,"about_ca_topic_score_gemma":0.0002952506,"domain_scores_codex":[0.9986163,0.000231806,0.0006623873,0.0002128947,0.00007694302,0.0001997128],"domain_scores_gemma":[0.9991521,0.00007873167,0.0002953016,0.0002939106,0.0001278882,0.00005209569],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00028366,0.0001017151,0.003264418,0.00001307094,0.00003484379,0.00001835445,0.0001297729,4.899073e-7,0.9947503,0.0002730977,0.00005260795,0.001077682],"study_design_scores_gemma":[0.0005490819,0.0006885732,0.1158996,0.000006690965,0.00003806766,0.0002192432,0.0001490306,0.000004675886,0.8774367,0.0005036303,0.004402764,0.000101969],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9958597,0.0005206974,0.0001835255,0.002652628,0.0006132717,0.00009451356,0.00004121639,0.000002258457,0.00003218074],"genre_scores_gemma":[0.9974758,0.0007161819,0.001099917,0.0003329083,0.0003287286,0.000003776446,0.00002462321,0.00001080636,0.000007264719],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1173136,"threshold_uncertainty_score":0.4104564,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008282368964098867,"score_gpt":0.2512182623970928,"score_spread":0.2429358934329939,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}