{"id":"W2161902864","doi":"10.1128/jb.00141-10","title":"Membrane Topology and Identification of Critical Amino Acid Residues in the Wzx O-Antigen Translocase from <i>Escherichia coli</i> O157:H4","year":2010,"lang":"en","type":"article","venue":"Journal of Bacteriology","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":29,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"Canadian Institutes of Health Research","keywords":"Aspartic acid; Periplasmic space; Biology; Biochemistry; Membrane topology; Lysine; Amino acid; Cysteine; Threonine; Alanine; Peptide sequence; Serine; Escherichia coli; Transmembrane domain; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008531371,0.0001643195,0.0001737309,0.0001367012,0.00009593367,0.0002109852,0.000115923,0.0002519868,0.0002243163],"category_scores_gemma":[0.000180325,0.0001171816,0.0001846882,0.0001557193,0.0001564871,0.0001454168,0.0001339617,0.0002222156,0.0001493709],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001734141,"about_ca_system_score_gemma":0.0001112216,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000554689,"about_ca_topic_score_gemma":0.0005180323,"domain_scores_codex":[0.9999512,0.00000913133,0.000006057474,0.00001168285,0.0000138665,0.000007978629],"domain_scores_gemma":[0.9998896,0.00001332956,0.00005556939,0.000005613749,0.00001339999,0.00002250552],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009737229,0.00001088934,0.001369157,0.00001738767,0.000003492084,0.00008298652,0.00002968939,0.00009917015,0.997792,0.00002751498,0.000006533598,0.0004638479],"study_design_scores_gemma":[0.00002251001,0.0003365095,0.1128607,0.00001910715,0.0000449277,0.0008307532,0.00028768,0.00328306,0.8811666,0.0001088325,0.00102333,0.00001602205],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9992082,0.00009245174,0.0005544171,0.0000111327,0.000001816349,0.000003484556,0.0000611877,0.00000403932,0.00006341711],"genre_scores_gemma":[0.998062,0.0001229508,0.00129725,0.00001678297,0.000001620505,0.000006648458,0.0003077779,0.000004569849,0.0001803297],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.000554689,"threshold_uncertainty_score":0.001258254,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008282368964098867,"score_gpt":0.2512182623970928,"score_spread":0.2429358934329939,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}