{"id":"W2162331387","doi":"10.1093/glycob/cwq190","title":"Trapping and characterization of covalent intermediates of mutant retaining glycosyltransferases","year":2010,"lang":"en","type":"article","venue":"Glycobiology","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":82,"is_retracted":false,"has_abstract":true,"ca_institutions":"Alberta Glycomics Centre; University of Alberta","funders":"","keywords":"Chemistry; Glycosyltransferase; Anomer; Galactosyltransferase; Glycosyl; Glycosylation; Trisaccharide; Mutant; Covalent bond; Disaccharide; Enzyme; Stereochemistry; Glycosidic bond; Biochemistry; Organic chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002104534,0.0003107512,0.0001584743,0.0001124849,0.00008884441,0.0002404323,0.0002575849,0.0003080698,0.0003158079],"category_scores_gemma":[0.0003216385,0.0001262094,0.0001670319,0.0001182893,0.0001679646,0.0001584549,0.0001605665,0.0004583885,0.0001707031],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001973131,"about_ca_system_score_gemma":0.0001347527,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004562491,"about_ca_topic_score_gemma":0.0004423192,"domain_scores_codex":[0.9998882,0.00001738977,0.000007852669,0.00002903752,0.00003898664,0.00001858765],"domain_scores_gemma":[0.9998042,0.00003555248,0.00007376874,0.00003273262,0.00002311812,0.00003061392],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001730662,0.000005483755,0.0001253013,0.000005176209,0.000002541234,0.00001743685,0.00000750852,0.00003026566,0.9994677,0.00004175864,0.000004074521,0.0002755114],"study_design_scores_gemma":[0.000002465363,0.00005755819,0.0007138341,8.096464e-7,0.000004087457,0.00012285,0.000006734662,0.0003346607,0.998508,0.00001861523,0.0002286418,0.000001743647],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9929471,0.0002029334,0.006445972,0.00001946532,0.000005750236,0.0000153651,0.0001129139,0.00004816629,0.0002021515],"genre_scores_gemma":[0.9909281,0.0002424196,0.007745365,0.00001673241,0.00000253169,0.00001694414,0.0003443302,0.00002587673,0.0006777397],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0004562491,"threshold_uncertainty_score":0.001431584,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007796801313612216,"score_gpt":0.2474759876239376,"score_spread":0.2396791863103254,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}