{"id":"W2162331387","doi":"10.1093/glycob/cwq190","title":"Trapping and characterization of covalent intermediates of mutant retaining glycosyltransferases","year":2010,"lang":"en","type":"article","venue":"Glycobiology","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":82,"is_retracted":false,"has_abstract":true,"ca_institutions":"Alberta Glycomics Centre; University of Alberta","funders":"","keywords":"Chemistry; Glycosyltransferase; Anomer; Galactosyltransferase; Glycosyl; Glycosylation; Trisaccharide; Mutant; Covalent bond; Disaccharide; Enzyme; Stereochemistry; Glycosidic bond; Biochemistry; Organic chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002109991,0.00009981429,0.0001871231,0.00008713574,0.00003133323,0.000004698664,0.000113589,0.0001697055,0.00007541585],"category_scores_gemma":[0.0001507103,0.00008994704,0.00004888451,0.00007627257,0.0002709628,0.000004207712,0.00005744281,0.0001181419,0.000001326714],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000002092533,"about_ca_system_score_gemma":0.00005032992,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001834812,"about_ca_topic_score_gemma":0.00004577691,"domain_scores_codex":[0.9992102,0.00005918873,0.0002803279,0.0002163344,0.00006857282,0.0001653492],"domain_scores_gemma":[0.9995085,0.00002695844,0.0001003468,0.0001884061,0.0001242708,0.00005154674],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001819708,0.00003439893,0.01260727,0.00004490263,0.00002852168,0.000001059301,0.00008274033,7.55307e-7,0.9803442,0.0003428123,0.000004135306,0.006327283],"study_design_scores_gemma":[0.000495957,0.0004362725,0.02078724,0.00001553711,0.000007757838,0.00002760704,0.00003771997,0.00006670282,0.9741589,0.00004020922,0.003838077,0.00008803631],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9984502,0.00006933923,0.0007905044,0.0000924725,0.0001391988,0.0001790599,0.00006056985,0.00000634602,0.0002123312],"genre_scores_gemma":[0.9992021,0.0002359685,0.0001671133,0.00003682011,0.00007578737,0.00001264766,0.0002257759,0.000009943783,0.00003381976],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.008179968,"threshold_uncertainty_score":0.3667934,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007796801313612216,"score_gpt":0.2474759876239376,"score_spread":0.2396791863103254,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}