{"id":"W2163024465","doi":"10.1021/bi700199g","title":"Exploration of New Chromophore Structures Leads to the Identification of Improved Blue Fluorescent Proteins","year":2007,"lang":"en","type":"article","venue":"Biochemistry","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":324,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Canada Research Chairs; University of Alberta; Sanofi","keywords":"Green fluorescent protein; Chromophore; Fluorescence; Fluorescent protein; mCherry; Protein tag; Aequorea victoria; Chemistry; Protein engineering; Biophysics; Photochemistry; Biochemistry; Biology; Fusion protein; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002355878,0.0001412359,0.0001216735,0.00002963037,0.00004088214,0.000009483958,0.0003362212,0.0001656135,0.00000489865],"category_scores_gemma":[0.0001786397,0.0001167467,0.00006741692,0.0001479963,0.00010714,0.000006662837,0.0001024397,0.00007744903,0.000001059159],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002779359,"about_ca_system_score_gemma":0.0001062667,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002329136,"about_ca_topic_score_gemma":0.00001624669,"domain_scores_codex":[0.9989451,0.00001140929,0.0004286358,0.0002902322,0.000146378,0.0001782108],"domain_scores_gemma":[0.998903,0.000004928895,0.0002640518,0.0005934539,0.0001661338,0.00006837452],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001150848,0.00003099518,0.0001382686,0.00005243415,0.00001118044,2.394046e-7,0.0001502626,0.00002261216,0.9952067,0.00001686369,0.001090201,0.003165174],"study_design_scores_gemma":[0.0001335831,0.0001159736,0.0005058546,0.00002492658,0.00000786981,0.000002525254,0.0001714815,0.000009201793,0.9980653,0.00007351062,0.0007724614,0.0001172912],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.824567,0.0003791649,0.1743818,0.0001160538,0.00005780091,0.0004324832,0.00002942966,0.00001566221,0.00002061162],"genre_scores_gemma":[0.9822139,0.00004206514,0.01709459,0.00003973261,0.0002086745,0.00002146313,0.0001072548,0.00001913888,0.0002531447],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1576469,"threshold_uncertainty_score":0.4760793,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0113703983737031,"score_gpt":0.2910647849484478,"score_spread":0.2796943865747447,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}