{"id":"W2165534020","doi":"10.1073/pnas.0501976102","title":"Capturing protein interactions in the secretory pathway of living cells","year":2005,"lang":"en","type":"article","venue":"Proceedings of the National Academy of Sciences","topic":"Cellular transport and secretion","field":"Biochemistry, Genetics and Molecular Biology","cited_by":179,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal","funders":"Universität Basel; Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung; National Science Foundation","keywords":"Protein-fragment complementation assay; Secretory pathway; Bimolecular fluorescence complementation; Cell biology; Endoplasmic reticulum; Yellow fluorescent protein; Secretion; Calnexin; Secretory protein; Endoplasmic-reticulum-associated protein degradation; Protein–protein interaction; Transmembrane protein; Biology; Transport protein; Golgi apparatus; Complementation; Chemistry; Biochemistry; Unfolded protein response; Receptor; Gene; Mutant","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007974215,0.00004691349,0.00005872605,0.00005601264,0.00005540447,0.000005716345,0.0003550225,0.00003865262,0.000008580801],"category_scores_gemma":[0.0001176755,0.00002950601,0.0000545962,0.0001737023,0.0002248118,0.00001982532,0.00003594145,0.00008962995,2.158206e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00000806276,"about_ca_system_score_gemma":0.00002202184,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000008009523,"about_ca_topic_score_gemma":0.000001698689,"domain_scores_codex":[0.9992824,0.000006557034,0.0001957275,0.0001121488,0.0003324814,0.00007071959],"domain_scores_gemma":[0.9996994,0.00002505838,0.0001814362,0.000007640748,0.00007752717,0.000008935848],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00000512132,0.00002877102,0.002118533,0.0000225727,0.000003288465,1.327483e-9,0.0003670345,0.0001055142,0.9957619,0.001457419,0.00002219948,0.0001076261],"study_design_scores_gemma":[0.00003621844,0.00002041274,0.01926878,0.0001033864,0.000002852135,0.000001731785,0.0002973106,0.0001958666,0.9780426,0.0005543531,0.001439872,0.00003664571],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9965148,0.000118715,0.00001093355,0.0007432351,0.000008171792,0.0001226397,0.000004859618,0.000001191557,0.00247546],"genre_scores_gemma":[0.9993921,0.00001356483,0.0003360679,0.00006320932,0.00007217719,0.000007145716,6.130575e-7,0.000001886413,0.000113263],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01771934,"threshold_uncertainty_score":0.120322,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02452709554303806,"score_gpt":0.2654830182180276,"score_spread":0.2409559226749895,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}