{"id":"W2166563565","doi":"10.1093/protein/gzq027","title":"Epitope mapping in cell surface proteins by site-directed masking: defining the structural elements of NTPDase3 inhibition by a monoclonal antibody","year":2010,"lang":"en","type":"article","venue":"Protein Engineering Design and Selection","topic":"Adenosine and Purinergic Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval; Centre hospitalier universitaire de Québec","funders":"Canadian Institutes of Health Research; National Institutes of Health; National Heart, Lung, and Blood Institute; University of Cincinnati; American Society for Pharmacology and Experimental Therapeutics","keywords":"Epitope; Monoclonal antibody; Chemistry; Homology modeling; Epitope mapping; Binding site; Biotinylation; Molecular biology; Cysteine; Active site; Biochemistry; Antibody; Alanine scanning; Enzyme; Mutant; Biology; Mutagenesis; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000536532,0.0008093239,0.000621298,0.0002475647,0.0001761514,0.0003589836,0.000733301,0.0005965335,0.000710859],"category_scores_gemma":[0.0003932236,0.000305112,0.0005875256,0.0002215032,0.0003532245,0.0002486904,0.0004525047,0.001041631,0.0004313304],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004104821,"about_ca_system_score_gemma":0.0001952474,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005622638,"about_ca_topic_score_gemma":0.000531345,"domain_scores_codex":[0.9995678,0.00009706638,0.00003945169,0.00007910912,0.0001282622,0.00008838121],"domain_scores_gemma":[0.9997157,0.00007598403,0.00007690434,0.0000524134,0.00004290499,0.00003601747],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002178837,0.00001041934,0.00004640819,0.00001454162,0.000002617257,0.00002041817,0.000006924512,0.00006119091,0.999358,0.00005250439,0.000005763563,0.0003993904],"study_design_scores_gemma":[0.00001016162,0.0001059176,0.0003844853,0.000002360737,0.000007972882,0.0001583621,0.000004540374,0.001189384,0.9974155,0.00002794509,0.0006906671,0.000002703046],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.938518,0.001306554,0.05855767,0.000114568,0.00004493191,0.0001461174,0.0001428473,0.0001655653,0.001003648],"genre_scores_gemma":[0.9590149,0.000884734,0.03853513,0.0001043079,0.00001514054,0.000133554,0.0002569376,0.00005053469,0.001004795],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008093239,"threshold_uncertainty_score":0.002978265,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003858847926720416,"score_gpt":0.200732062096932,"score_spread":0.1968732141702116,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}