{"id":"W2166580635","doi":"10.1016/j.jmb.2006.03.004","title":"Kinetics and Energetics of Ligand Binding Determined by Microcalorimetry: Insights into Active Site Mobility in a Psychrophilic α-Amylase","year":2006,"lang":"en","type":"article","venue":"Journal of Molecular Biology","topic":"thermodynamics and calorimetric analyses","field":"Chemistry","cited_by":59,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Fonds De La Recherche Scientifique - FNRS; Polar Knowledge Canada","keywords":"Psychrophile; Isothermal titration calorimetry; Enzyme kinetics; Active site; Chemistry; Isothermal microcalorimetry; Ligand (biochemistry); Hydrolysis; Biochemistry; Enzyme; Stereochemistry; Thermodynamics; Enthalpy","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00010224,0.0001577047,0.0004206318,0.0002756158,0.00002019836,0.000008980629,0.0001544489,0.0002103763,0.000009190103],"category_scores_gemma":[0.00008017077,0.0001331348,0.0001164699,0.0003063261,0.0001694388,0.00002911848,0.00005742039,0.0002154854,3.527039e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00007706016,"about_ca_system_score_gemma":0.00003126925,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001650249,"about_ca_topic_score_gemma":0.00003940416,"domain_scores_codex":[0.9989101,0.00005388618,0.0005760147,0.0001848037,0.0001085726,0.0001666088],"domain_scores_gemma":[0.9990923,0.00009752507,0.0004656743,0.0001548337,0.0001206913,0.00006896042],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001268339,0.0002762325,0.005663356,0.00003367629,0.00006612134,0.00004211472,0.00006273969,0.00004404787,0.9914526,0.00006115617,0.00001192526,0.002159155],"study_design_scores_gemma":[0.0009991464,0.0002670485,0.0009572952,0.00003365755,0.00007090998,0.00003309863,0.00005485196,0.0003095842,0.9944287,0.002339379,0.0003654787,0.0001408451],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9924181,0.005265366,0.002066838,0.0000307301,0.00004743703,0.00002784448,0.00001701707,0.000003008778,0.0001236341],"genre_scores_gemma":[0.9989344,0.0003446221,0.0006042627,0.000020229,0.00005026601,8.57716e-7,0.00001786364,0.00001397303,0.00001347922],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.006516322,"threshold_uncertainty_score":0.5429078,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004330466877677205,"score_gpt":0.2444579983072412,"score_spread":0.240127531429564,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}