{"id":"W2166749737","doi":"10.1093/glycob/cwt001","title":"Structure-based mutagenic analysis of mechanism and substrate specificity in mammalian glycosyltransferases: Porcine ST3Gal-I","year":2013,"lang":"en","type":"article","venue":"Glycobiology","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":22,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph; University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Michael Smith Health Research BC","keywords":"Sialyltransferase; Chemistry; Biochemistry; Glycosyltransferase; Substrate (aquarium); Neuraminic acid; Function (biology); Stereochemistry; Substrate specificity; Mutant; Transferase; Acceptor; Amino acid; Active site; Enzyme; Sialic acid; Biology; Cell biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.0002158084,0.0002147538,0.000417169,0.0004481466,0.00004423405,0.00001977933,0.0002125794,0.0003023356,0.001149413],"category_scores_gemma":[0.00006440802,0.0001930084,0.0001289314,0.0005898229,0.0001798014,0.000007025693,0.00005716022,0.0001650294,0.00001021579],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001816562,"about_ca_system_score_gemma":0.00008117481,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008385073,"about_ca_topic_score_gemma":0.002474401,"domain_scores_codex":[0.9983491,0.0001907195,0.0004249089,0.000514372,0.0001290464,0.0003919086],"domain_scores_gemma":[0.9991909,0.00003386694,0.00009326721,0.0004140318,0.0001445751,0.0001233459],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002271596,0.00006808899,0.0405384,0.00002642659,0.0002051096,0.000008344948,0.00002774461,0.0003575911,0.9569923,0.0008861654,0.00004407995,0.0006185527],"study_design_scores_gemma":[0.001786721,0.0006914055,0.2230502,0.000007750147,0.0001126274,0.00001101197,0.00009336961,0.002031493,0.7703845,0.0009591086,0.0005503795,0.0003214872],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9971428,0.0002038039,0.00155763,0.0001869957,0.0000616376,0.0004678076,0.0001524683,0.000009586934,0.0002172736],"genre_scores_gemma":[0.998516,0.00006171053,0.0004650046,0.0001468458,0.00004153925,0.00003426681,0.0005912295,0.00001616848,0.0001271892],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1866079,"threshold_uncertainty_score":0.9997637,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009142230374614794,"score_gpt":0.2438540049302801,"score_spread":0.2347117745556654,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}