{"id":"W2166749737","doi":"10.1093/glycob/cwt001","title":"Structure-based mutagenic analysis of mechanism and substrate specificity in mammalian glycosyltransferases: Porcine ST3Gal-I","year":2013,"lang":"en","type":"article","venue":"Glycobiology","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":22,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph; University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Michael Smith Health Research BC","keywords":"Sialyltransferase; Chemistry; Biochemistry; Glycosyltransferase; Substrate (aquarium); Neuraminic acid; Function (biology); Stereochemistry; Substrate specificity; Mutant; Transferase; Acceptor; Amino acid; Active site; Enzyme; Sialic acid; Biology; Cell biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002196248,0.0003130345,0.0001061502,0.0001339134,0.0000855237,0.0001520617,0.0002322958,0.0002763831,0.0005088884],"category_scores_gemma":[0.0002184948,0.0001059169,0.0002457851,0.0001172353,0.0002116731,0.0001000797,0.0001081928,0.0003728372,0.0001262636],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003405295,"about_ca_system_score_gemma":0.000202088,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005463061,"about_ca_topic_score_gemma":0.0006050899,"domain_scores_codex":[0.9998997,0.00002965172,0.00001078016,0.00001912208,0.00002835881,0.00001232366],"domain_scores_gemma":[0.9998676,0.0000553207,0.00003995625,0.00001594072,0.00001155124,0.000009672667],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005391198,0.00001917728,0.0002402597,0.00001782198,0.00000634036,0.00001855403,0.000005972326,0.0004152772,0.9985169,0.0001664325,0.00000582955,0.0005335322],"study_design_scores_gemma":[0.000009264916,0.0001527173,0.001519875,0.000002116886,0.00001266646,0.0001089262,0.000005202701,0.002345866,0.9954609,0.00004812862,0.0003319494,0.000002366275],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9923205,0.0002721727,0.006511322,0.00003325794,0.000007740179,0.0000246759,0.0001570499,0.00004317575,0.0006301475],"genre_scores_gemma":[0.9955983,0.0002538582,0.00353609,0.00001741014,0.000001878338,0.00001782403,0.0002412982,0.00001588032,0.0003173136],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005463061,"threshold_uncertainty_score":0.002470791,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009142230374614794,"score_gpt":0.2438540049302801,"score_spread":0.2347117745556654,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}