{"id":"W2166825525","doi":"10.1128/iai.68.7.4344-4348.2000","title":"Mechanical Fractionation Reveals Structural Requirements for Enteropathogenic <i>Escherichia coli</i> Tir Insertion into Host Membranes","year":2000,"lang":"en","type":"article","venue":"Infection and Immunity","topic":"Escherichia coli research studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":46,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"Biology; Enteropathogenic Escherichia coli; Intimin; Escherichia coli; Lysis; Secretion; HeLa; Transmembrane protein; Microbiology; Cell membrane; Membrane; Cell fractionation; Bacterial outer membrane; Cell; Molecular biology; Enterobacteriaceae; Biochemistry; Receptor","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001518508,0.0002615167,0.0001615461,0.0001310336,0.0002458125,0.0005033239,0.0002181054,0.0002820758,0.0006218638],"category_scores_gemma":[0.0003287384,0.0001967064,0.0001407002,0.0001125436,0.0002788491,0.0003091151,0.000251805,0.0003974883,0.0004038564],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005751456,"about_ca_system_score_gemma":0.0001682507,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006812127,"about_ca_topic_score_gemma":0.001163186,"domain_scores_codex":[0.9998606,0.00002393471,0.00001233476,0.00002483235,0.00003684692,0.00004149208],"domain_scores_gemma":[0.9998071,0.00004931071,0.00007377951,0.00001729548,0.00002718007,0.00002541832],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000284984,0.000005538694,0.0001281883,0.000009078032,8.455714e-7,0.00001714644,0.000007466288,0.00001515872,0.999619,0.00004336677,0.000006463876,0.0001192478],"study_design_scores_gemma":[0.000006842658,0.00008388435,0.0113993,0.000006834995,0.000008978906,0.0002077708,0.00009332842,0.0007730689,0.9862216,0.00007226884,0.001121463,0.000004844519],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9981268,0.0002932717,0.0009256066,0.00007286436,0.000004133684,0.000005760273,0.0000494529,0.00001267308,0.0005094034],"genre_scores_gemma":[0.9978368,0.000275019,0.0008911228,0.00005647678,0.000005637257,0.00001281486,0.0003339137,0.0000162503,0.0005719987],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006812127,"threshold_uncertainty_score":0.004172981,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01819570376381171,"score_gpt":0.3046225760964911,"score_spread":0.2864268723326794,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}