{"id":"W2167290062","doi":"10.1006/prep.2001.1483","title":"High-Level Expression and Purification of Immunogenic Recombinant SAG1 (P30) of Toxoplasma gondii in Escherichia coli","year":2001,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Toxoplasma gondii Research Studies","field":"Immunology and Microbiology","cited_by":45,"is_retracted":false,"has_abstract":false,"ca_institutions":"Royal University Hospital","funders":"","keywords":"Toxoplasma gondii; Recombinant DNA; Immunogenicity; Escherichia coli; Biology; Fusion protein; Lysis; Antigen; Virology; Microbiology; Molecular biology; Gene; Antibody; Biochemistry; Immunology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006486122,0.0002274948,0.0004345349,0.0002842706,0.0001852687,0.00001526412,0.0002175626,0.0003348938,0.0001059923],"category_scores_gemma":[0.0003067903,0.0001900615,0.00004885692,0.0003009256,0.0003353274,0.000216016,0.0001636077,0.0003292192,0.00001518035],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004132781,"about_ca_system_score_gemma":0.00005762338,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001985376,"about_ca_topic_score_gemma":0.00002192196,"domain_scores_codex":[0.997945,0.0004681053,0.0006905277,0.000462439,0.0001152531,0.0003186756],"domain_scores_gemma":[0.9987299,0.0001610747,0.0004741405,0.0004344927,0.0001575873,0.00004276835],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0007431814,0.0003142656,0.002870427,0.0001429057,0.00002565753,0.000001021289,0.0009018471,0.000003501537,0.9833528,0.0005989658,0.0002250834,0.0108203],"study_design_scores_gemma":[0.001955431,0.0002218706,0.06343025,0.0004164032,0.00001211974,0.000008874434,0.0004132474,0.00001867357,0.9294027,0.0005775143,0.003363345,0.0001795584],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9901983,0.007619806,0.000308622,0.0003750375,0.0001370547,0.001155187,0.00003749067,0.00003292623,0.0001356441],"genre_scores_gemma":[0.9925227,0.003537131,0.002228229,0.000007906373,0.00001121315,0.0003518812,0.00009502121,0.00001966902,0.001226216],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.06055982,"threshold_uncertainty_score":0.7750483,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02521626350113841,"score_gpt":0.2619384869001085,"score_spread":0.2367222233989701,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}