{"id":"W2167290062","doi":"10.1006/prep.2001.1483","title":"High-Level Expression and Purification of Immunogenic Recombinant SAG1 (P30) of Toxoplasma gondii in Escherichia coli","year":2001,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Toxoplasma gondii Research Studies","field":"Immunology and Microbiology","cited_by":45,"is_retracted":false,"has_abstract":false,"ca_institutions":"Royal University Hospital","funders":"","keywords":"Toxoplasma gondii; Recombinant DNA; Immunogenicity; Escherichia coli; Biology; Fusion protein; Lysis; Antigen; Virology; Microbiology; Molecular biology; Gene; Antibody; Biochemistry; Immunology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004460648,0.0006586676,0.0005925343,0.0004077793,0.0003192693,0.0005112513,0.0004200658,0.0003682837,0.0005265797],"category_scores_gemma":[0.0004359602,0.0002692036,0.0003758834,0.0007933427,0.0002580165,0.0003122167,0.0003546921,0.0009394116,0.001028364],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005213574,"about_ca_system_score_gemma":0.0003611164,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001746899,"about_ca_topic_score_gemma":0.001728866,"domain_scores_codex":[0.9996454,0.00007587469,0.00004405115,0.00004095483,0.0001204622,0.00007318291],"domain_scores_gemma":[0.9997151,0.00006987373,0.00003988403,0.00005300976,0.00007341997,0.00004861709],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006049961,0.00004478182,0.0001469271,0.00005339509,0.000004917595,0.00006606871,0.00002896075,0.0001007813,0.9983951,0.0001169308,0.0001073295,0.0008742868],"study_design_scores_gemma":[0.00001594393,0.0001028179,0.002295643,0.000009686718,0.00001284676,0.0002662715,0.00004011171,0.001431008,0.9921253,0.0001099059,0.003583493,0.000006911128],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9479135,0.002088249,0.04029308,0.0006391557,0.0001737603,0.0003526469,0.004971865,0.0004705967,0.003097158],"genre_scores_gemma":[0.9207364,0.002651782,0.04686429,0.0002330502,0.00004754764,0.0002697758,0.01856312,0.0002084105,0.0104256],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001746899,"threshold_uncertainty_score":0.003782749,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02521626350113841,"score_gpt":0.2619384869001085,"score_spread":0.2367222233989701,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}