{"id":"W2167783675","doi":"10.1110/ps.072894407","title":"A rapid and universal tandem‐purification strategy for recombinant proteins","year":2007,"lang":"en","type":"article","venue":"Protein Science","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":27,"is_retracted":false,"has_abstract":true,"ca_institutions":"University Health Network; University of Toronto; Ontario Institute for Cancer Research","funders":"Canadian Breast Cancer Research Alliance","keywords":"Tandem affinity purification; Recombinant DNA; FLAG-tag; Fusion protein; Protein purification; Escherichia coli; Myc-tag; Affinity chromatography; Tandem; Green fluorescent protein; Chemistry; Biochemistry; Chromatography; Tandem repeat; Biology; Computational biology; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006316316,0.0006355225,0.0005729003,0.0003136628,0.0002839223,0.0004104772,0.0008062234,0.0004820229,0.001004392],"category_scores_gemma":[0.000431234,0.000310311,0.0004953798,0.0003205115,0.0003779078,0.000441111,0.0005831698,0.001557478,0.001666261],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000444035,"about_ca_system_score_gemma":0.0005517169,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004078127,"about_ca_topic_score_gemma":0.0005113142,"domain_scores_codex":[0.9994248,0.0001074198,0.0000503112,0.0001512507,0.0001996945,0.00006664165],"domain_scores_gemma":[0.9998158,0.00002930219,0.00003688514,0.00004426051,0.00004746463,0.0000264274],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002215986,0.00002821732,0.00005333612,0.00007910175,0.00000851431,0.00004004971,0.00001908564,0.00006651615,0.9914926,0.0004320109,0.0003214525,0.007437161],"study_design_scores_gemma":[0.00001531125,0.000121442,0.0003289579,0.000007808417,0.00001544391,0.0005649954,0.000009072264,0.0009589851,0.9823254,0.0001641432,0.01547661,0.00001170784],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1925272,0.005409322,0.7933458,0.0008105086,0.0002957058,0.001012596,0.0007704535,0.002116697,0.0037116],"genre_scores_gemma":[0.4147606,0.005969966,0.5635421,0.0005787042,0.0001006707,0.00101882,0.003188747,0.0003005114,0.01054001],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001004392,"threshold_uncertainty_score":0.003360033,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06488664452267986,"score_gpt":0.3534983339572313,"score_spread":0.2886116894345514,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}