{"id":"W2167783675","doi":"10.1110/ps.072894407","title":"A rapid and universal tandem‐purification strategy for recombinant proteins","year":2007,"lang":"en","type":"article","venue":"Protein Science","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":27,"is_retracted":false,"has_abstract":true,"ca_institutions":"University Health Network; University of Toronto; Ontario Institute for Cancer Research","funders":"Canadian Breast Cancer Research Alliance","keywords":"Tandem affinity purification; Recombinant DNA; FLAG-tag; Fusion protein; Protein purification; Escherichia coli; Myc-tag; Affinity chromatography; Tandem; Green fluorescent protein; Chemistry; Biochemistry; Chromatography; Tandem repeat; Biology; Computational biology; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001575079,0.00008280713,0.0001176219,0.0001538702,0.0002922327,0.00004191417,0.0001383835,0.00003956747,0.00003597869],"category_scores_gemma":[0.0001973519,0.00006168337,0.00002986191,0.0004020956,0.0005792583,0.0001633611,0.00004850697,0.0001147385,0.000009090142],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005901155,"about_ca_system_score_gemma":0.0003078327,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00008273524,"about_ca_topic_score_gemma":0.00001194304,"domain_scores_codex":[0.9987355,0.00001202079,0.0001523974,0.0003158213,0.0003991703,0.000385019],"domain_scores_gemma":[0.9993055,0.000045309,0.00004733866,0.0001515621,0.000225087,0.0002251905],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000598882,0.0001423205,0.002339127,0.0001504363,0.000009250864,0.00002247529,0.0002266044,4.441318e-7,0.7116162,0.02844028,0.00008707718,0.2563669],"study_design_scores_gemma":[0.002885109,0.004067742,0.1326803,0.0002837935,0.00002539414,0.0001780754,0.0006688444,0.00299613,0.7711536,0.007879476,0.07676596,0.0004155221],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9833505,0.0004619526,0.005557616,0.002612557,0.00005374895,0.002426907,0.00000387307,0.0000343293,0.005498572],"genre_scores_gemma":[0.9860718,0.00005146999,0.009354364,0.00005800959,0.00006699213,0.00005866711,0.000003382218,0.000006272961,0.004329105],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2559514,"threshold_uncertainty_score":0.2515375,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06488664452267986,"score_gpt":0.3534983339572313,"score_spread":0.2886116894345514,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}