{"id":"W2168535398","doi":"10.1038/emboj.2011.377","title":"Structure and activity of the Cas3 HD nuclease MJ0384, an effector enzyme of the CRISPR interference","year":2011,"lang":"en","type":"article","venue":"The EMBO Journal","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":148,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto","funders":"Argonne National Laboratory; Natural Sciences and Engineering Research Council of Canada; Government of Canada; National Institutes of Health; Genome Canada; Ontario Genomics; National Institute of General Medical Sciences; Ontario Genomics Institute","keywords":"Biology; Nuclease; Effector; CRISPR; Enzyme; Interference (communication); Genetics; CRISPR interference; RNA interference; Computational biology; Molecular biology; Cell biology; Biochemistry; Gene; RNA; Genome editing","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001539942,0.00008377252,0.00009003602,0.000009373043,0.00008374971,0.000009286849,0.0003364965,0.00004563601,0.00003829489],"category_scores_gemma":[0.00005069783,0.00003856246,0.00006863596,0.0000374244,0.0001179075,0.000003414066,0.000141254,0.000186324,1.988931e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000003810455,"about_ca_system_score_gemma":0.00002870573,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003186795,"about_ca_topic_score_gemma":0.00004633582,"domain_scores_codex":[0.9995195,0.0001049576,0.000109095,0.00008149512,0.00008405086,0.0001009571],"domain_scores_gemma":[0.9994828,0.00001073701,0.0001023307,0.000320803,0.00004218662,0.00004118953],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006598362,0.00001925799,0.002341764,0.0000151149,0.00003564386,5.20149e-7,0.0005914927,0.00008638841,0.9948196,0.00001654051,0.0001317194,0.001875976],"study_design_scores_gemma":[0.0001639163,0.0001261371,0.08731079,0.00002206542,0.00003193953,0.0001321808,0.00008077936,0.00009961474,0.9117072,0.0001517172,0.0001233375,0.00005029767],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9983555,0.0003628399,0.0008034142,0.00005538898,0.0002255925,0.00007282355,0.00001417553,0.000001314653,0.000108924],"genre_scores_gemma":[0.9997103,0.00003921706,0.00006415804,0.00003541398,0.0001062759,5.751388e-7,2.679558e-7,0.000008279747,0.00003546932],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.08496902,"threshold_uncertainty_score":0.1572532,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01139437633006212,"score_gpt":0.2648367254169601,"score_spread":0.2534423490868979,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}