{"id":"W2168535398","doi":"10.1038/emboj.2011.377","title":"Structure and activity of the Cas3 HD nuclease MJ0384, an effector enzyme of the CRISPR interference","year":2011,"lang":"en","type":"article","venue":"The EMBO Journal","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":148,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto","funders":"Argonne National Laboratory; Natural Sciences and Engineering Research Council of Canada; Government of Canada; National Institutes of Health; Genome Canada; Ontario Genomics; National Institute of General Medical Sciences; Ontario Genomics Institute","keywords":"Biology; Nuclease; Effector; CRISPR; Enzyme; Interference (communication); Genetics; CRISPR interference; RNA interference; Computational biology; Molecular biology; Cell biology; Biochemistry; Gene; RNA; Genome editing","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001660276,0.0001306453,0.0002502672,0.000189524,0.0002869123,0.0005096202,0.00051112,0.0004516564,0.001347524],"category_scores_gemma":[0.000384902,0.0002102079,0.0002568715,0.0002389537,0.0003075085,0.0002261116,0.000161497,0.0007734332,0.0006892006],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008686764,"about_ca_system_score_gemma":0.0003665849,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00211248,"about_ca_topic_score_gemma":0.001910329,"domain_scores_codex":[0.999846,0.00001697828,0.00001342079,0.0000373442,0.00006188901,0.00002434473],"domain_scores_gemma":[0.9998598,0.00003250889,0.0000333908,0.00002508035,0.00001845083,0.00003071546],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003237058,0.00007027654,0.0006075033,0.00003553913,0.000006994253,0.00006054341,0.00002885629,0.0006492397,0.9940637,0.0007068595,0.0002829097,0.003163971],"study_design_scores_gemma":[0.00002193511,0.00007874563,0.007271258,0.00000483066,0.00001041274,0.0001908748,0.00001690971,0.004374812,0.9843245,0.0001995407,0.003490773,0.00001540831],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9898852,0.001032931,0.004055188,0.0002302684,0.00003269962,0.00001164611,0.0009687186,0.0001543357,0.003629108],"genre_scores_gemma":[0.991055,0.0002628681,0.00318599,0.00006813699,0.000004770228,0.000008516723,0.001987108,0.00004728705,0.003380426],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00211248,"threshold_uncertainty_score":0.006302714,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01139437633006212,"score_gpt":0.2648367254169601,"score_spread":0.2534423490868979,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}