{"id":"W2168696688","doi":"10.1016/j.jviromet.2006.05.009","title":"Transfection of Arabidopsis protoplasts with a Plum pox virus (PPV) infectious clone for studying early molecular events associated with PPV infection","year":2006,"lang":"en","type":"article","venue":"Journal of Virological Methods","topic":"Plant Virus Research Studies","field":"Agricultural and Biological Sciences","cited_by":12,"is_retracted":false,"has_abstract":false,"ca_institutions":"Western University; Agriculture and Agri-Food Canada","funders":"Agriculture and Agri-Food Canada; Natural Sciences and Engineering Research Council of Canada; Canadian Food Inspection Agency","keywords":"Biology; Protoplast; Arabidopsis; Transfection; Virus; Virology; Molecular biology; RNA; Potyvirus; Viral replication; Cell culture; Gene; Plant virus; Genetics; Mutant","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003488837,0.0005551528,0.0004682419,0.0005188032,0.0006134327,0.0005232847,0.0004795593,0.0005320632,0.002618904],"category_scores_gemma":[0.0002919448,0.0004794544,0.0006809228,0.0005508707,0.0003506704,0.0005951449,0.0004773403,0.002178497,0.0009075596],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008549714,"about_ca_system_score_gemma":0.0004031993,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001536189,"about_ca_topic_score_gemma":0.002249756,"domain_scores_codex":[0.9997556,0.00003828339,0.00002230551,0.00005499486,0.00006612929,0.00006278101],"domain_scores_gemma":[0.9996313,0.0001743427,0.00004112302,0.00005167159,0.00003043299,0.00007123271],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004140742,0.00001720635,0.00004353745,0.00002402975,0.000002554435,0.00003175035,0.00003657993,0.00001692846,0.99927,0.0002021154,0.00001982491,0.0002939929],"study_design_scores_gemma":[0.00003121688,0.0001604752,0.003556556,0.000007317374,0.00002962748,0.0001668429,0.00006401142,0.0008309407,0.9915418,0.00009676013,0.00350376,0.00001074363],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.9415685,0.001525192,0.04425467,0.0003486469,0.0004269627,0.0005278547,0.002697714,0.0006389745,0.008011505],"genre_scores_gemma":[0.9393674,0.001301364,0.02682279,0.0001878466,0.0000895367,0.0004833943,0.009398354,0.0003488409,0.02200042],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.002618904,"threshold_uncertainty_score":0.008761108,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03753360968208528,"score_gpt":0.3187351797018987,"score_spread":0.2812015700198134,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}