{"id":"W2169279655","doi":"10.1186/1746-4811-8-44","title":"FACS-based purification of Arabidopsis microspores, sperm cells and vegetative nuclei","year":2012,"lang":"en","type":"article","venue":"Plant Methods","topic":"Plant Reproductive Biology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":94,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Fundação para a Ciência e a Tecnologia; Natural Sciences and Engineering Research Council of Canada; Fred C. Gloeckner Foundation","keywords":"Microspore; Arabidopsis; Biology; Mitosis; Cell biology; Germ cell; Sperm; Pollen tube; Double fertilization; Egg cell; Cell cycle; Epigenetics; Genetics; Pollen; Cell; Botany; Gene; Stamen","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002581794,0.0005017512,0.0001769229,0.0003503003,0.0003587336,0.0002495933,0.0003580277,0.0002107562,0.001864594],"category_scores_gemma":[0.0001644028,0.0001090866,0.0001786038,0.000204817,0.0002043463,0.0001939748,0.0002306888,0.0005121725,0.001292369],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003397156,"about_ca_system_score_gemma":0.0003613039,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001082285,"about_ca_topic_score_gemma":0.002007711,"domain_scores_codex":[0.9998872,0.00001343385,0.00001215405,0.00002890217,0.00004045183,0.00001784731],"domain_scores_gemma":[0.9998811,0.00002660145,0.00001543305,0.00001565759,0.00003953582,0.00002160527],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002573428,0.0000104729,0.0002070234,0.00004578457,0.000003982837,0.00002426934,0.00001497966,0.00006359645,0.9973462,0.0001468347,0.0001214895,0.001989747],"study_design_scores_gemma":[0.00002121167,0.00009551942,0.004097737,0.00001383818,0.00002004855,0.0002545975,0.00002181289,0.001741094,0.9848015,0.0002093703,0.008714088,0.000009055675],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.717642,0.003150039,0.2558883,0.0006147725,0.0001871491,0.001088818,0.01088311,0.001778031,0.008767813],"genre_scores_gemma":[0.7396922,0.002937971,0.2196581,0.0003415054,0.00007946219,0.0009542354,0.02470894,0.000396262,0.01123134],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001864594,"threshold_uncertainty_score":0.006237626,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02426290133213383,"score_gpt":0.314631324466792,"score_spread":0.2903684231346582,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}