{"id":"W2170721683","doi":"10.1002/cbic.200200540","title":"Large‐Scale In Vivo Synthesis of the Carbohydrate Moieties of Gangliosides GM1 and GM2 by Metabolically Engineered <i>Escherichia coli</i>","year":2003,"lang":"en","type":"article","venue":"ChemBioChem","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":79,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph; Institute for Biological Sciences","funders":"","keywords":"Biochemistry; Sialyltransferase; Escherichia coli; Oligosaccharide; Sialic acid; Uridine diphosphate glucose; Uridine diphosphate; Transferase; Galactosyltransferase; Glycosyltransferase; Glycan; Cytidine; Chemistry; Nucleotide salvage; Glycolipid; Biology; Enzyme; Gene; Glycoprotein","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001397754,0.0007518869,0.0002899216,0.0001151783,0.0001108071,0.0003376475,0.0002967383,0.0002619822,0.0006200756],"category_scores_gemma":[0.00007838472,0.0001512036,0.0002510029,0.0001750689,0.0001576058,0.0001715393,0.0002541016,0.000512928,0.0005306949],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003009605,"about_ca_system_score_gemma":0.0002615103,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008712262,"about_ca_topic_score_gemma":0.0009314659,"domain_scores_codex":[0.9999121,0.00001636053,0.000008194214,0.00002025339,0.00002286039,0.0000201314],"domain_scores_gemma":[0.9999393,0.000008141796,0.00002187738,0.000009480925,0.000007771193,0.00001340728],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002732349,0.00001088566,0.00005132063,0.00001835331,0.00000285015,0.00001130781,0.000003035567,0.00005947374,0.9992679,0.00004908715,0.00002352325,0.0004749163],"study_design_scores_gemma":[0.000005383027,0.00005846066,0.0004594344,0.000001822807,0.00001070729,0.00006982041,0.000005415487,0.0004564174,0.9971219,0.00001208085,0.001796353,0.000002232372],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9714385,0.001374109,0.02155521,0.0001361287,0.00005741415,0.0001095234,0.001413088,0.0003383633,0.003577616],"genre_scores_gemma":[0.9478748,0.001654906,0.04032406,0.0001085273,0.00001596701,0.00007900138,0.00364934,0.0001630947,0.006130284],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008712262,"threshold_uncertainty_score":0.002183616,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004535406663083223,"score_gpt":0.2115663260903762,"score_spread":0.207030919427293,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}