{"id":"W2171045920","doi":"10.1021/ja711248y","title":"Probing the Substrate Specificity of Golgi α-Mannosidase II by Use of Synthetic Oligosaccharides and a Catalytic Nucleophile Mutant","year":2008,"lang":"en","type":"article","venue":"Journal of the American Chemical Society","topic":"Carbohydrate Chemistry and Synthesis","field":"Chemistry","cited_by":61,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; Ontario Institute for Cancer Research","funders":"National Center for Research Resources; National Institute of General Medical Sciences","keywords":"Chemistry; Mannosidase; Mutant; Nucleophile; Substrate (aquarium); Glycoside hydrolase; Catalysis; Substrate specificity; Golgi apparatus; Stereochemistry; Biochemistry; Combinatorial chemistry; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002060272,0.0001993818,0.0005495929,0.000008996044,0.0001702928,0.00001803212,0.0004934417,0.00007591768,0.00004094063],"category_scores_gemma":[0.0002471617,0.0001187514,0.0005734022,0.000246806,0.001679853,0.0001197587,0.0001852839,0.0004591011,2.434799e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00009325283,"about_ca_system_score_gemma":0.00008295284,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001051526,"about_ca_topic_score_gemma":3.698844e-7,"domain_scores_codex":[0.9985191,0.00002827179,0.0006548965,0.0001807986,0.0003847138,0.0002322651],"domain_scores_gemma":[0.9976137,0.0004519885,0.001287553,0.0004005775,0.0001393389,0.0001068354],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000786142,0.0001733972,0.001227934,0.00009552617,0.0001703933,0.000005071506,0.00111893,0.00001552874,0.9954023,0.000003665851,0.001573491,0.0001351683],"study_design_scores_gemma":[0.0001970119,0.00002297051,0.0002468363,0.0001499623,0.0001377755,0.0002801674,0.0009113768,0.0001541478,0.9974452,0.00005847577,0.0002654733,0.0001305551],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9987629,0.000173166,0.00000381841,0.0008209731,0.00001925171,0.00004516245,0.00008356966,0.000007446807,0.00008369568],"genre_scores_gemma":[0.9992346,0.0003541011,0.0001521663,0.00008631194,0.00008336634,0.000001976815,0.000001504179,0.00001886843,0.00006707916],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002042967,"threshold_uncertainty_score":0.6189489,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01919903192198256,"score_gpt":0.2139733255830134,"score_spread":0.1947742936610309,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}