{"id":"W2171170097","doi":"","title":"Solid-phase PCR with hybridization and time-resolved fluorometry for detection of HLA-B27.","year":2001,"lang":"en","type":"article","venue":"PubMed","topic":"Advanced Biosensing Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"Juvenile Diabetes Research Foundation","funders":"","keywords":"Microtiter plate; Oligonucleotide; Primer dimer; Molecular biology; Chemistry; Primer (cosmetics); genomic DNA; Linker; Polymerase chain reaction; Fluorescence; Multiplex polymerase chain reaction; DNA; Chromatography; Biology; Biochemistry; Gene; Organic chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002994404,0.002611511,0.00136088,0.002050589,0.0004141444,0.0007586392,0.001889183,0.001846417,0.004455865],"category_scores_gemma":[0.002336294,0.001601656,0.001308251,0.001332938,0.001376618,0.0008934193,0.0009864446,0.002494028,0.004351587],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007140515,"about_ca_system_score_gemma":0.0006302326,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002894347,"about_ca_topic_score_gemma":0.000685138,"domain_scores_codex":[0.9927481,0.002542932,0.000399769,0.002124209,0.00189434,0.0002907354],"domain_scores_gemma":[0.9979984,0.001004633,0.0003112842,0.0002888214,0.0003126858,0.00008427744],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001187543,0.00008212435,0.0001841086,0.0003336334,0.00002929566,0.00005477876,0.00004463406,0.0002152827,0.9879569,0.0002310915,0.0001628311,0.01058652],"study_design_scores_gemma":[0.00002977283,0.0005021029,0.001087569,0.00003453739,0.00006608063,0.0006451625,0.00003537102,0.009895931,0.9831388,0.0002645172,0.004256377,0.00004382446],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.04480509,0.003723376,0.9462895,0.0001394337,0.0002932353,0.0005916103,0.0003967273,0.002212446,0.001548554],"genre_scores_gemma":[0.08860166,0.003122312,0.8997635,0.0001959376,0.0001156303,0.001944702,0.001113825,0.000256907,0.0048857],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.004455865,"threshold_uncertainty_score":0.01583612,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01142153454034467,"score_gpt":0.2851488525517699,"score_spread":0.2737273180114252,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}