{"id":"W2179585078","doi":"10.1371/journal.pone.0139637","title":"A TaqMan-Based Multiplex qPCR Assay and DNA Extraction Method for Phylotype IIB Sequevars 1&2 (Select Agent) Strains of Ralstonia solanacearum","year":2015,"lang":"en","type":"article","venue":"PLoS ONE","topic":"Plant Pathogenic Bacteria Studies","field":"Agricultural and Biological Sciences","cited_by":15,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Animal and Plant Health Inspection Service; Oak Ridge Associated Universities; Agricultural Research Service; Oak Ridge Institute for Science and Education; U.S. Department of Agriculture; U.S. Department of Energy","keywords":"Ralstonia solanacearum; TaqMan; Biology; Phylotype; Multiplex; Biovar; DNA extraction; Multiplex polymerase chain reaction; Geranium; Microbiology; Bacterial wilt; Polymerase chain reaction; Pathogen; Genetics; Botany; Gene; 16S ribosomal RNA; Cultivar","routes":{"ca_aff":false,"ca_fund":false,"ca_venue":false,"about_ca":true,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007167507,0.0001563185,0.0003330134,0.0000155132,0.0001090005,0.0000260249,0.0001182914,0.000102978,0.00003745055],"category_scores_gemma":[0.0004592067,0.00007242371,0.00006756356,0.0001482226,0.00005098404,0.00008547261,0.00003476359,0.00008637866,0.000008519735],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004283641,"about_ca_system_score_gemma":0.00002600502,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001467086,"about_ca_topic_score_gemma":0.001821683,"domain_scores_codex":[0.9988012,0.0001745003,0.0002263698,0.0002900778,0.0002329812,0.0002748638],"domain_scores_gemma":[0.9990402,0.0004662397,0.0001669843,0.00006154387,0.000151633,0.0001133539],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001431383,0.0003656281,0.002731365,0.0000358892,0.0001129078,0.00000109506,0.00008471851,0.000002996398,0.9924434,0.00001716902,0.0002937068,0.003768027],"study_design_scores_gemma":[0.002521751,0.002045411,0.3381767,0.0002098823,0.0005823674,0.00001402958,0.0004575075,0.007267592,0.6391055,0.0005438991,0.008199972,0.0008753954],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9974163,0.0001601211,0.0003728334,0.0007824393,0.00004411574,0.0005084857,0.0005291752,0.00006182945,0.0001247454],"genre_scores_gemma":[0.9744201,0.00002484702,0.02479632,0.00008584518,0.0001298599,0.00005502214,0.0002056668,0.000002558596,0.0002798209],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.3533378,"threshold_uncertainty_score":0.2953353,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1731209392666856,"score_gpt":0.2917617518162159,"score_spread":0.1186408125495304,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}