{"id":"W2179585078","doi":"10.1371/journal.pone.0139637","title":"A TaqMan-Based Multiplex qPCR Assay and DNA Extraction Method for Phylotype IIB Sequevars 1&2 (Select Agent) Strains of Ralstonia solanacearum","year":2015,"lang":"en","type":"article","venue":"PLoS ONE","topic":"Plant Pathogenic Bacteria Studies","field":"Agricultural and Biological Sciences","cited_by":15,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Animal and Plant Health Inspection Service; Oak Ridge Associated Universities; Agricultural Research Service; Oak Ridge Institute for Science and Education; U.S. Department of Agriculture; U.S. Department of Energy","keywords":"Ralstonia solanacearum; TaqMan; Biology; Phylotype; Multiplex; Biovar; DNA extraction; Multiplex polymerase chain reaction; Geranium; Microbiology; Bacterial wilt; Polymerase chain reaction; Pathogen; Genetics; Botany; Gene; 16S ribosomal RNA; Cultivar","routes":{"ca_aff":false,"ca_fund":false,"ca_venue":false,"about_ca":true,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002910555,0.00225417,0.001618493,0.002099668,0.0007063181,0.0009386668,0.002011199,0.001718875,0.005894342],"category_scores_gemma":[0.002798439,0.001885023,0.001287766,0.001639298,0.0009970327,0.0008172059,0.001066718,0.002324614,0.003566652],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001113244,"about_ca_system_score_gemma":0.00206686,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009013948,"about_ca_topic_score_gemma":0.002725932,"domain_scores_codex":[0.9909025,0.001482679,0.0008259721,0.004073245,0.001991776,0.0007237935],"domain_scores_gemma":[0.9982151,0.0005197438,0.0003079449,0.0003169662,0.000465518,0.000174714],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001786513,0.0002678591,0.002265096,0.0002637225,0.00004271522,0.00004697482,0.0001496198,0.0005994084,0.9842184,0.001079642,0.001101478,0.00978639],"study_design_scores_gemma":[0.0001382096,0.00185711,0.0102016,0.0001186616,0.000176192,0.0004017071,0.0001265166,0.02215994,0.9256958,0.0009149585,0.03807629,0.0001330827],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1903686,0.001157757,0.7845017,0.000509879,0.000815972,0.003199288,0.008057373,0.004574005,0.006815348],"genre_scores_gemma":[0.1365554,0.0007159607,0.809055,0.001142931,0.0002127005,0.01118449,0.01904842,0.0004787518,0.02160628],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.005894342,"threshold_uncertainty_score":0.01971853,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1731209392666856,"score_gpt":0.2917617518162159,"score_spread":0.1186408125495304,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}