{"id":"W2180687425","doi":"10.1128/aem.02776-15","title":"MiL-FISH: Multilabeled Oligonucleotides for Fluorescence <i>In Situ</i> Hybridization Improve Visualization of Bacterial Cells","year":2015,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Microbial Community Ecology and Physiology","field":"Environmental Science","cited_by":58,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Max-Planck-Gesellschaft; Gordon and Betty Moore Foundation","keywords":"Fluorescence in situ hybridization; Oligonucleotide; Fish <Actinopterygii>; Biology; Oligomer restriction; In situ; Computational biology; Cell sorting; Isolation (microbiology); Chemistry; Microbiology; Biochemistry; DNA; Cell; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002069159,0.0001586785,0.0002552284,0.00003360996,0.00005995026,0.000004599326,0.0001564139,0.0001652211,0.0001702247],"category_scores_gemma":[0.000009783392,0.0001611814,0.0000292241,0.0000565195,0.0004690144,0.00006593107,0.0002325047,0.0000845214,0.00002847085],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00007864439,"about_ca_system_score_gemma":0.000007606113,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00006279645,"about_ca_topic_score_gemma":0.0002221913,"domain_scores_codex":[0.9990435,0.00009081738,0.0002821533,0.0003029297,0.00002581767,0.0002548214],"domain_scores_gemma":[0.9995716,0.0000797037,0.0001294937,0.0001565789,0.000002406096,0.00006024767],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002145282,0.0001682236,0.00189138,0.00001092003,0.000007682382,3.526804e-7,0.0003890866,0.0001086086,0.9955749,0.00006153763,0.0008713476,0.0007014408],"study_design_scores_gemma":[0.002127454,0.0002986146,0.005989872,0.000004618481,0.00001781702,0.000006641136,0.0002437214,0.000120167,0.984502,0.0002680731,0.006187249,0.0002337728],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9988015,0.00001420652,0.0002032737,0.00002515905,0.0001498399,0.0004729328,0.0001003183,0.0000171605,0.0002155555],"genre_scores_gemma":[0.9977592,0.0000571495,0.001108522,0.0002403898,0.00002527849,0.00002970178,0.0007349647,0.00001951777,0.00002524955],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01107289,"threshold_uncertainty_score":0.6572785,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007934341270646583,"score_gpt":0.2014219283481442,"score_spread":0.1934875870774976,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}