{"id":"W2181486621","doi":"10.21769/bioprotoc.1139","title":"Small-scale Triton X-114 Extraction of Hydrophobic Proteins","year":2014,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Prion Diseases and Protein Misfolding","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"National Institute of Neurological Disorders and Stroke; Alberta Prion Research Institute; National Institutes of Health","keywords":"Chemistry; Chromatography; Guanidine; Hydrophobic effect; Extraction (chemistry); Protein purification; Hydrophilic interaction chromatography; Proteases; Phase (matter); Hydrochloride; Aqueous solution; Enzyme; Biochemistry; Organic chemistry; High-performance liquid chromatography","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009163163,0.002368019,0.00105895,0.001977702,0.001576525,0.001151519,0.001656505,0.001025093,0.01180351],"category_scores_gemma":[0.001028074,0.001111045,0.0008232787,0.001184742,0.0006324237,0.001004333,0.000850847,0.002589193,0.01572976],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001056725,"about_ca_system_score_gemma":0.001763403,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001650302,"about_ca_topic_score_gemma":0.005867193,"domain_scores_codex":[0.9991993,0.0001062135,0.0001102045,0.0002213292,0.0002561062,0.0001068718],"domain_scores_gemma":[0.999512,0.0001102225,0.00004605633,0.0001627859,0.0001094321,0.00005954843],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0001790712,0.0001503745,0.0001882273,0.0005600757,0.00003811915,0.00015124,0.00009407906,0.0001344346,0.9724086,0.001244586,0.004789159,0.02006195],"study_design_scores_gemma":[0.00009866652,0.0002820301,0.002815661,0.0001066082,0.0001114532,0.0007003877,0.00005093071,0.001636459,0.9063662,0.001049795,0.08672874,0.00005307731],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1618296,0.01545919,0.7154315,0.001729842,0.002492914,0.009936541,0.02429882,0.01077291,0.05804877],"genre_scores_gemma":[0.1686286,0.01533097,0.4853997,0.0009257875,0.0003051628,0.0121382,0.1153175,0.002175834,0.1997783],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01180351,"threshold_uncertainty_score":0.03948665,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01551504813620602,"score_gpt":0.2913640635125107,"score_spread":0.2758490153763047,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}