{"id":"W2181486621","doi":"10.21769/bioprotoc.1139","title":"Small-scale Triton X-114 Extraction of Hydrophobic Proteins","year":2014,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Prion Diseases and Protein Misfolding","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"National Institute of Neurological Disorders and Stroke; Alberta Prion Research Institute; National Institutes of Health","keywords":"Chemistry; Chromatography; Guanidine; Hydrophobic effect; Extraction (chemistry); Protein purification; Hydrophilic interaction chromatography; Proteases; Phase (matter); Hydrochloride; Aqueous solution; Enzyme; Biochemistry; Organic chemistry; High-performance liquid chromatography","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002504481,0.0001666049,0.0001616224,0.00004795973,0.00007879705,0.00002168585,0.0002129771,0.0001827289,0.000121656],"category_scores_gemma":[0.00005593479,0.0001522144,0.0001519258,0.00009458298,0.00005987939,0.000004830513,0.00008757295,0.00007911823,0.00002128419],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001367929,"about_ca_system_score_gemma":0.0000638604,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005098964,"about_ca_topic_score_gemma":0.00001997132,"domain_scores_codex":[0.9988913,0.0001046505,0.0002975972,0.0003415257,0.0001413272,0.0002236102],"domain_scores_gemma":[0.9991596,0.000009115572,0.0001956289,0.0004518137,0.00007412616,0.0001097528],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003737104,0.0001397813,0.0008471106,0.0001638454,0.00001422313,6.764827e-7,0.000007376364,0.000009986621,0.9660934,0.00002997078,0.0004597214,0.03186024],"study_design_scores_gemma":[0.00106896,0.0004757786,0.001240879,0.00003587049,0.000007858968,0.000004069037,0.000006641465,0.00008751682,0.672141,0.00008610693,0.3246941,0.0001512441],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"protocol","genre_gemma":"protocol","genre_scores_codex":[0.3337504,0.00006494756,0.00476905,0.0001431187,0.0001279323,0.6575868,0.00002554366,0.00006233554,0.003469906],"genre_scores_gemma":[0.2490879,0.000005273937,0.002194774,0.00009096143,0.0004350894,0.7477107,0.00002504364,0.00003169099,0.0004185085],"genre_candidate":"protocol","genre_consensus":"protocol","teacher_disagreement_score":0.3242344,"threshold_uncertainty_score":0.6207124,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01551504813620602,"score_gpt":0.2913640635125107,"score_spread":0.2758490153763047,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}