{"id":"W2208298605","doi":"10.1038/mt.2015.190","title":"Silent IL2RG Gene Editing in Human Pluripotent Stem Cells","year":2015,"lang":"en","type":"article","venue":"Molecular Therapy","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":7,"is_retracted":false,"has_abstract":false,"ca_institutions":"Princess Margaret Cancer Centre; Sunnybrook Hospital; University of Toronto; University Health Network","funders":"National Institute of Diabetes and Digestive and Kidney Diseases; National Heart, Lung, and Blood Institute; Canadian Institutes of Health Research; National Institutes of Health","keywords":"Induced pluripotent stem cell; Biology; Chromatin; Homologous recombination; Genetics; Gene targeting; Transgene; Genome editing; Gene knockin; Selectable marker; Human artificial chromosome; Gene; Retrotransposon; CRISPR; Cell biology; Genome; Embryonic stem cell; Transposable element; Chromosome","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005642914,0.0003383923,0.0003164278,0.0002591298,0.0001909151,0.0006299054,0.0005483086,0.0005851118,0.001929728],"category_scores_gemma":[0.0002582833,0.0002152597,0.0002728303,0.0001348474,0.0004055562,0.0002515372,0.000318529,0.0008577352,0.0006954325],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003126334,"about_ca_system_score_gemma":0.000272102,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002447302,"about_ca_topic_score_gemma":0.0005258697,"domain_scores_codex":[0.9995396,0.0000946316,0.00005652975,0.0001009198,0.0001448033,0.00006363319],"domain_scores_gemma":[0.9998158,0.00006182926,0.00004155861,0.00004503167,0.0000164956,0.00001938299],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004402542,0.000009864809,0.00006400391,0.00003473953,0.000004640364,0.00008404037,0.000021835,0.0001440375,0.9964114,0.00107735,0.00009779984,0.002006303],"study_design_scores_gemma":[0.00001282926,0.00003404772,0.0001845915,0.000003555281,0.00001055715,0.0001819537,0.000009498523,0.0005933814,0.9953122,0.0001378308,0.003516283,0.000003345814],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8368533,0.002727619,0.1416001,0.00093778,0.0004277557,0.0002750407,0.001744462,0.001723387,0.01371048],"genre_scores_gemma":[0.9733739,0.0005733205,0.01697777,0.0001764999,0.00003021978,0.00009000964,0.0005907051,0.0001744881,0.008013265],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001929728,"threshold_uncertainty_score":0.0064556,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01594564522027632,"score_gpt":0.2857658430656295,"score_spread":0.2698201978453532,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}