{"id":"W2212369523","doi":"10.1021/acschembio.5b00774","title":"Brighter Red Fluorescent Proteins by Rational Design of Triple-Decker Motif","year":2015,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":25,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Canada Foundation for Innovation; Ontario Ministry of Economic Development and Innovation; Canada Research Chairs; Government of Canada","keywords":"Chromophore; Quantum yield; Chemistry; Steric effects; Fluorescence; Rational design; Biophysics; Photochemistry; Stereochemistry; Crystallography; Nanotechnology; Materials science; Biology; Physics; Optics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000188266,0.0001858755,0.0002308296,0.00002941983,0.0000215357,0.00000570468,0.0003058012,0.0003669205,0.0000199517],"category_scores_gemma":[0.0003242885,0.000159752,0.00005885796,0.00007938395,0.0003278053,0.000005383885,0.0001588141,0.0001250711,0.00000810604],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003437714,"about_ca_system_score_gemma":0.0001007336,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000007295397,"about_ca_topic_score_gemma":4.324583e-7,"domain_scores_codex":[0.9987743,0.00009338653,0.000324725,0.0004205347,0.00009822788,0.0002888334],"domain_scores_gemma":[0.9992189,0.00001595903,0.0001256469,0.0003417235,0.0001797536,0.0001179765],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002907229,0.0001028962,0.0005692489,0.000005306336,0.00002327305,7.109928e-7,0.00001175385,0.00000191103,0.9628981,0.00009974415,0.03526615,0.0007302377],"study_design_scores_gemma":[0.0006661194,0.0003481872,0.00001575357,0.000008264076,0.000007585776,0.000008675675,0.000005655047,0.00002595803,0.9812072,0.001188416,0.0163407,0.0001775175],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.4644949,0.001377697,0.5311894,0.0007746484,0.0002364566,0.001327012,0.0001700891,0.0001014435,0.0003284051],"genre_scores_gemma":[0.857891,0.000092311,0.1400409,0.000367696,0.0001778383,0.0001296978,0.0009948572,0.00003636,0.0002693341],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.3933962,"threshold_uncertainty_score":0.6514497,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01967031841111637,"score_gpt":0.2852953137458217,"score_spread":0.2656249953347053,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}