{"id":"W2213167196","doi":"10.1016/j.dib.2015.04.024","title":"Polymerase chain reaction-based gene removal from plasmids","year":2015,"lang":"en","type":"article","venue":"Data in Brief","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"University of Illinois at Urbana-Champaign; Carnegie Institution for Science; McMaster University","keywords":"Restriction enzyme; Plasmid; Polymerase chain reaction; Multiplex polymerase chain reaction; Restriction digest; Cloning (programming); Multiplex; Restriction site; Gene; Molecular biology; Genetics; DNA; Biology; Computational biology; Computer science","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.004716045,0.005063514,0.004682474,0.003395035,0.001737856,0.001912586,0.004846879,0.001875562,0.01794285],"category_scores_gemma":[0.004637286,0.003612044,0.003481336,0.002951412,0.002000551,0.001100123,0.002785813,0.00883857,0.03130672],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001054975,"about_ca_system_score_gemma":0.002030305,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008447189,"about_ca_topic_score_gemma":0.002376986,"domain_scores_codex":[0.9861273,0.002323099,0.002176534,0.005457141,0.002656176,0.001259725],"domain_scores_gemma":[0.9961905,0.001094477,0.0003789933,0.001392957,0.0006730146,0.000270056],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003329674,0.0004128857,0.0002970097,0.001458894,0.0001175072,0.0003761995,0.0003931906,0.0006708498,0.9460089,0.004715082,0.01026114,0.03495527],"study_design_scores_gemma":[0.0002077734,0.0004720069,0.0005984732,0.0001865414,0.0002155165,0.0006114661,0.00005785654,0.003308311,0.8798175,0.001117253,0.1132624,0.0001448443],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.01293125,0.001433376,0.9553293,0.0003213529,0.0009506699,0.006482267,0.006724407,0.009814561,0.00601287],"genre_scores_gemma":[0.04984804,0.002832318,0.8715613,0.0005919441,0.0002281759,0.01170116,0.03661121,0.002735984,0.02388975],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01794285,"threshold_uncertainty_score":0.0600248,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02624619194917641,"score_gpt":0.3084759309957976,"score_spread":0.2822297390466211,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}