{"id":"W2221183325","doi":"10.1016/j.pep.2015.12.015","title":"Improving the soluble expression and purification of recombinant human stem cell factor (SCF) in endotoxin-free Escherichia coli by disulfide shuffling with persulfide","year":2015,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Health Sciences Centre Foundation","keywords":"Thioredoxin; Escherichia coli; Biochemistry; Fusion protein; Molecular biology; Affinity chromatography; Protein disulfide-isomerase; Expression vector; Biology; Inclusion bodies; Recombinant DNA; Chemistry; Enzyme; Gene","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004539157,0.0004370498,0.0002945982,0.0002698113,0.0001476549,0.0003088927,0.0002693972,0.0002107932,0.0004303881],"category_scores_gemma":[0.0003315371,0.0001723185,0.0003411641,0.0003561995,0.000190464,0.0002702487,0.0002561971,0.0005939566,0.0003368918],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003080745,"about_ca_system_score_gemma":0.0004035031,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009996383,"about_ca_topic_score_gemma":0.001177981,"domain_scores_codex":[0.9997011,0.00005390133,0.00004231789,0.00003327819,0.0001309764,0.00003839703],"domain_scores_gemma":[0.9998622,0.00002365198,0.00003374816,0.00001764849,0.00004606329,0.00001677355],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002996343,0.00001952813,0.000142359,0.00003517079,0.000005582682,0.00002886952,0.00001740685,0.0001126166,0.9974039,0.0001760182,0.00005584165,0.001972709],"study_design_scores_gemma":[0.00000427655,0.00003198423,0.0003733132,0.000002182678,0.000007569519,0.00008426827,0.000007660799,0.000721014,0.9971506,0.00004029401,0.001574255,0.00000248019],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.9094675,0.001465509,0.08662119,0.0003369183,0.00005066681,0.00008865629,0.0005456307,0.0003521018,0.001071946],"genre_scores_gemma":[0.9232068,0.001996117,0.06828725,0.000109201,0.00001992926,0.00005705857,0.002162899,0.0001399455,0.004020737],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.0009996383,"threshold_uncertainty_score":0.002400577,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01912460548817338,"score_gpt":0.2342458772613087,"score_spread":0.2151212717731354,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}