{"id":"W2221625412","doi":"10.1016/j.ab.2015.11.020","title":"Development of a high-throughput liquid state assay for lipase activity using natural substrates and rhodamine B","year":2015,"lang":"en","type":"article","venue":"Analytical Biochemistry","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":22,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université du Québec à Trois-Rivières; PROTEO; Université Laval","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Triolein; Lipase; Substrate (aquarium); Chromatography; Rhodamine B; Rhodamine; Chemistry; Emulsion; Fluorescence; Enzyme; Biochemistry; Biology; Catalysis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002626404,0.0001454553,0.000195783,0.00002051138,0.0000418428,0.00001716188,0.00008425723,0.0001188508,0.000002683242],"category_scores_gemma":[0.0002787551,0.0001322462,0.0000592693,0.00009062923,0.0001093786,0.000007110671,0.00008741027,0.00005457335,4.781462e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003055438,"about_ca_system_score_gemma":0.0001994813,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001139054,"about_ca_topic_score_gemma":0.000009566097,"domain_scores_codex":[0.9991193,0.00001663339,0.000234585,0.0003289041,0.0001183821,0.000182209],"domain_scores_gemma":[0.9993366,0.00002029345,0.0001014257,0.0002050157,0.000212675,0.0001240186],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002478982,0.00008062241,0.0001326679,0.00006533763,0.00008578256,5.833626e-7,0.00002843147,0.00002783498,0.9985701,0.00001357813,0.0001508212,0.0005963339],"study_design_scores_gemma":[0.000498945,0.0001096296,0.0001223095,0.00001579782,0.00004725511,0.000005007692,0.0000577963,0.002288506,0.9961581,0.00001816815,0.0005123252,0.0001661725],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9925414,0.0003799946,0.00684583,0.00003896914,0.00003344422,0.00008847747,0.00002987733,0.00000580803,0.00003618152],"genre_scores_gemma":[0.9935888,0.00001538447,0.005670494,0.00002856437,0.00007312421,0.000007484873,0.0003514975,0.00001313981,0.0002515339],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002412021,"threshold_uncertainty_score":0.5392845,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02656083516924467,"score_gpt":0.294622246782877,"score_spread":0.2680614116136323,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}