{"id":"W2222298501","doi":"10.2527/jas.2011-4884","title":"Cryopreservation of green fluorescent protein (GFP)-labeled primordial germ cells with GFP fused to the 3′ untranslated region of the nanos gene by vitrification of Japanese eel (Anguilla japonica) somite stage embryos1","year":2012,"lang":"en","type":"article","venue":"Journal of Animal Science","topic":"Animal Genetics and Reproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"Vancouver Island University","funders":"","keywords":"Green fluorescent protein; Cryoprotectant; Vitrification; Biology; Cryopreservation; Embryo; Molecular biology; Chemistry; Cell biology; Andrology; Gene; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009194527,0.0001120887,0.0001762031,0.00005801031,0.0001006188,0.00001265777,0.0005250667,0.00007757948,0.000003098418],"category_scores_gemma":[0.00009866229,0.00006177673,0.00008783856,0.0004790298,0.0003407592,0.00002652547,0.00007926214,0.0001024417,3.068251e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002413743,"about_ca_system_score_gemma":0.0002270492,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000105496,"about_ca_topic_score_gemma":0.00001422528,"domain_scores_codex":[0.9984791,0.00009396535,0.0004679847,0.0001981939,0.0005505452,0.0002102531],"domain_scores_gemma":[0.9980332,0.000008234209,0.0008405439,0.000368411,0.0006617022,0.00008793645],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0009996447,0.0001548825,0.001168706,0.00002588676,0.00002343279,2.364196e-7,0.0004698187,0.0001608791,0.9965659,0.00001309784,0.00009701415,0.0003204999],"study_design_scores_gemma":[0.0003883943,0.001290656,0.06006875,0.00003553916,0.00003090866,0.00001339373,0.0001413434,0.00008439156,0.9373783,0.000001317288,0.0004914561,0.0000755642],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9981526,0.0004297422,0.0002417726,0.0005698112,0.0000937732,0.0004645404,0.00001129624,0.000001364888,0.00003506249],"genre_scores_gemma":[0.9988644,0.00006324024,0.0006601812,0.00002702036,0.0001536236,0.000003492035,0.000002053793,0.000009682136,0.0002162956],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.05918761,"threshold_uncertainty_score":0.2519182,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01101619251950623,"score_gpt":0.2320730571273804,"score_spread":0.2210568646078742,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}