{"id":"W2234521185","doi":"10.1101/pdb.top084111","title":"Construction of Multifragment Plasmids by Homologous Recombination in Yeast","year":2015,"lang":"en","type":"article","venue":"Cold Spring Harbor Protocols","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Canadian Institutes of Health Research; Howard Hughes Medical Institute","keywords":"Homologous recombination; Plasmid; Cloning (programming); Saccharomyces cerevisiae; Genetics; Yeast; Genome; In vitro recombination; FLP-FRT recombination; Biology; Computational biology; DNA; Homologous chromosome; Insert (composites); Gene; Molecular cloning; Recombination; Genetic recombination; Computer science; Peptide sequence","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001445356,0.0007393048,0.0009485431,0.001231326,0.0007261193,0.001038719,0.001679142,0.0007174425,0.003082191],"category_scores_gemma":[0.0007043939,0.0007004017,0.0007558041,0.001186618,0.0005064273,0.0005775165,0.001327437,0.002104728,0.004000138],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005769625,"about_ca_system_score_gemma":0.0006181099,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006835512,"about_ca_topic_score_gemma":0.0008065092,"domain_scores_codex":[0.9991635,0.000139917,0.0001395324,0.0001804118,0.0002722968,0.0001043902],"domain_scores_gemma":[0.9996136,0.00009617992,0.00007438989,0.00009454154,0.0000528829,0.00006848243],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002507098,0.0001434877,0.0002698509,0.0005111316,0.00002906878,0.000346007,0.0002485565,0.00112245,0.9632857,0.007278182,0.001960592,0.02455432],"study_design_scores_gemma":[0.0001662961,0.0004717767,0.001792975,0.0001151432,0.00006871636,0.001327809,0.000110167,0.008495172,0.8458613,0.001820625,0.1396908,0.00007935861],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2918698,0.004817299,0.6612968,0.0009529836,0.0006491224,0.002918117,0.01342892,0.009041734,0.01502522],"genre_scores_gemma":[0.4824912,0.006227902,0.4409869,0.000308401,0.0001063677,0.001885584,0.0400892,0.002311435,0.02559293],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.003082191,"threshold_uncertainty_score":0.01031095,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02352963597839297,"score_gpt":0.2938453227505889,"score_spread":0.270315686772196,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}