{"id":"W2235568143","doi":"10.1139/cjm-2015-0602","title":"Rapid detection of <i>Cronobacter sakazakii</i> by real-time PCR based on the <i>cgcA</i> gene and TaqMan probe with internal amplification control","year":2015,"lang":"en","type":"article","venue":"Canadian Journal of Microbiology","topic":"Enterobacteriaceae and Cronobacter Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Doctoral Program Foundation of Institutions of Higher Education of China; Fundamental Research Funds for the Central Universities; National Natural Science Foundation of China","keywords":"Cronobacter sakazakii; Bacillus cereus; Microbiology; TaqMan; Biology; Real-time polymerase chain reaction; Detection limit; Cronobacter; Food microbiology; Bacteria; Chemistry; Infant formula; Food science; Gene; Enterobacter; Escherichia coli; Chromatography","routes":{"ca_aff":false,"ca_fund":false,"ca_venue":true,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004277645,0.0001343046,0.0001942095,0.0000870448,0.00005659402,0.00003140073,0.0002289179,0.0001252139,0.00002714747],"category_scores_gemma":[0.00004487597,0.00008989371,0.00005467692,0.00005139119,0.000255044,0.000007805959,0.00001367646,0.0001571368,0.000004177139],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005244881,"about_ca_system_score_gemma":0.0004625634,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005837941,"about_ca_topic_score_gemma":0.001887832,"domain_scores_codex":[0.9990317,0.0002281678,0.0002684808,0.0001758583,0.00004853697,0.000247304],"domain_scores_gemma":[0.9989881,0.00002580005,0.0002475963,0.0002171574,0.0002630045,0.0002583212],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006684254,0.00002104093,0.003979858,0.000005476859,0.00007051863,0.000004840575,0.00003627401,0.000007668353,0.99155,0.000002600377,0.002825558,0.0008276988],"study_design_scores_gemma":[0.002260264,0.003875937,0.002123579,0.00004372515,0.00003910865,0.0004414696,0.00007166344,0.0000168407,0.9507161,0.000008287311,0.04024563,0.0001574333],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9970455,0.000306977,0.001488575,0.0006580924,0.00008150349,0.0001782502,0.0001214622,0.0000015536,0.0001180434],"genre_scores_gemma":[0.9989542,0.00004872834,0.0001307535,0.0005699262,0.00009750589,0.000005927946,0.00006761962,0.00001670737,0.000108659],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.04083398,"threshold_uncertainty_score":0.3665759,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008347290436531814,"score_gpt":0.1989700572266559,"score_spread":0.1906227667901241,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}