{"id":"W2254619066","doi":"10.1093/protein/gzv044","title":"Development and characterization of cyclodextrin glucanotransferase as a maltoheptaose-producing enzyme using site-directed mutagenesis","year":2015,"lang":"en","type":"article","venue":"Protein Engineering Design and Selection","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Hallym University; National Research Foundation","keywords":"Saturated mutagenesis; Chemistry; Hydrolysis; Cyclodextrin; Enzyme kinetics; Residue (chemistry); Mutagenesis; Enzyme; Site-directed mutagenesis; Stereochemistry; Mutant; Substrate (aquarium); Active site; Directed evolution; Biochemistry; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002440947,0.0001283658,0.0001108999,0.00009684128,0.00005360296,0.00002375466,0.00002554213,0.00008934861,0.000002558077],"category_scores_gemma":[0.0000660283,0.0001421616,0.00001314798,0.0001647141,0.00001267263,0.00001770456,0.00001575678,0.00004947542,6.222382e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002407306,"about_ca_system_score_gemma":0.00007338135,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001616739,"about_ca_topic_score_gemma":0.000001648586,"domain_scores_codex":[0.9992869,0.00004530165,0.000186191,0.0002676672,0.00009123431,0.0001226366],"domain_scores_gemma":[0.99969,0.000002530618,0.00005818314,0.00006548042,0.0001093233,0.00007448554],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009488934,0.00002338511,0.0002021957,0.00006048238,0.00002047899,3.852476e-7,0.0003148659,0.001664968,0.9954917,0.00001194863,0.000001480779,0.002113259],"study_design_scores_gemma":[0.0003071489,0.00009991296,0.001496297,0.00003213601,0.00001294442,0.00003078549,0.00001027506,0.02397921,0.9729128,0.000001939611,0.0009617901,0.000154751],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7481147,0.0001143113,0.2514006,0.00001731699,0.00004301931,0.0002648323,0.000001095676,0.00004245681,0.000001622173],"genre_scores_gemma":[0.9697202,0.00003969771,0.02988446,0.00001246484,0.0001025429,0.00003595055,0.00005855248,0.0000244162,0.0001217429],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2216055,"threshold_uncertainty_score":0.5797183,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01780422075069099,"score_gpt":0.2145165928902465,"score_spread":0.1967123721395556,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}