{"id":"W2254619066","doi":"10.1093/protein/gzv044","title":"Development and characterization of cyclodextrin glucanotransferase as a maltoheptaose-producing enzyme using site-directed mutagenesis","year":2015,"lang":"en","type":"article","venue":"Protein Engineering Design and Selection","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Hallym University; National Research Foundation","keywords":"Saturated mutagenesis; Chemistry; Hydrolysis; Cyclodextrin; Enzyme kinetics; Residue (chemistry); Mutagenesis; Enzyme; Site-directed mutagenesis; Stereochemistry; Mutant; Substrate (aquarium); Active site; Directed evolution; Biochemistry; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002920347,0.0003831824,0.0003268637,0.0001836865,0.00009454913,0.0003335356,0.0002910938,0.0002831641,0.0003188149],"category_scores_gemma":[0.0003088429,0.0001710415,0.0003004164,0.0003021732,0.0001850011,0.0001376847,0.000164102,0.0004292375,0.0003755182],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002922927,"about_ca_system_score_gemma":0.0003060399,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001217037,"about_ca_topic_score_gemma":0.0008071517,"domain_scores_codex":[0.9997759,0.00004385553,0.00004263184,0.00004402906,0.00006444347,0.00002908393],"domain_scores_gemma":[0.9998004,0.00004182157,0.00004749293,0.00003313919,0.00004031664,0.00003696077],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001697688,0.00001918,0.0001441027,0.00001049363,0.000002538618,0.00002891987,0.000007207689,0.0001013099,0.9989578,0.00003538072,0.000005268292,0.000670772],"study_design_scores_gemma":[0.000004416268,0.00005700028,0.0007432221,0.000001343172,0.000007629354,0.00007888643,0.000008318305,0.0007955965,0.9977442,0.00001099517,0.0005458498,0.000002542559],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9763879,0.0003175887,0.02182054,0.0001121319,0.00001819184,0.0001231662,0.0004539046,0.00009704742,0.0006696227],"genre_scores_gemma":[0.9615848,0.0005050977,0.03479136,0.00003914348,0.000006279055,0.0000668519,0.001539341,0.00006105754,0.00140608],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001217037,"threshold_uncertainty_score":0.002419829,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01780422075069099,"score_gpt":0.2145165928902465,"score_spread":0.1967123721395556,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}