{"id":"W2256581714","doi":"10.1101/pdb.prot078337","title":"Murine Stem Cell–Based Retrovirus Production for Marking Primary Mouse Mammary Cells for Metastasis Studies","year":2016,"lang":"en","type":"article","venue":"Cold Spring Harbor Protocols","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Université de Montréal; Montreal Clinical Research Institute","funders":"","keywords":"Retrovirus; Biology; Green fluorescent protein; Transactivation; Transgene; Long terminal repeat; Oncogene; Viral vector; Gene; Reporter gene; Cell culture; Gene expression; In vivo; Cancer research; Genetically modified mouse; Molecular biology; Genetics; Recombinant DNA; Cell cycle","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002304936,0.001602185,0.000741544,0.003272302,0.001003251,0.0008391908,0.00180978,0.001371067,0.008936333],"category_scores_gemma":[0.0006131323,0.001183436,0.001065244,0.001206035,0.0007083805,0.0006104885,0.0006413813,0.002996199,0.008325714],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009505987,"about_ca_system_score_gemma":0.001031324,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007313438,"about_ca_topic_score_gemma":0.001836795,"domain_scores_codex":[0.9982242,0.0003856594,0.0002632916,0.0002817072,0.0006157241,0.0002294051],"domain_scores_gemma":[0.998912,0.0001987928,0.0002016291,0.0003170611,0.0001655181,0.0002050924],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009425922,0.0001146914,0.0001375158,0.000129232,0.00001251589,0.00007036318,0.00007997525,0.0002124488,0.9925697,0.001271843,0.0005971127,0.00471011],"study_design_scores_gemma":[0.0001037156,0.000505222,0.0009163041,0.00006976331,0.00005150798,0.0003329888,0.00002527503,0.001725612,0.9515983,0.0003784682,0.04426399,0.00002876946],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2581593,0.008963415,0.6490458,0.001363837,0.002153449,0.01649114,0.02768737,0.00676294,0.02937274],"genre_scores_gemma":[0.3546084,0.009764899,0.5429932,0.0004653992,0.000341941,0.01083612,0.03280414,0.00167592,0.04651011],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.008936333,"threshold_uncertainty_score":0.02989507,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04936208564753215,"score_gpt":0.3251092621366059,"score_spread":0.2757471764890738,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}