{"id":"W2258692498","doi":"10.1007/978-1-60327-015-1_26","title":"Use of Site-Specific Protein-DNA Photocrosslinking of Purified Complexes to Analyze the Topology of the RNA Polymerase II Transcription Initiation Complex","year":2009,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"RNA and protein synthesis mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"Montreal Clinical Research Institute","funders":"Canadian Institutes of Health Research","keywords":"DNA; Nucleotide; Transcription (linguistics); Molecular biology; Chemistry; Polymerase; DNA sequencing; Electrophoretic mobility shift assay; Biochemistry; Biology; Transcription factor; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003980865,0.0006389469,0.0003756554,0.0006329456,0.000484305,0.0005666426,0.0006140057,0.0004336894,0.001196265],"category_scores_gemma":[0.0004421871,0.0004268405,0.0004261522,0.0003383621,0.0004243871,0.0003865355,0.0003345429,0.001663903,0.0005757196],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006920779,"about_ca_system_score_gemma":0.0002848167,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008097212,"about_ca_topic_score_gemma":0.001413455,"domain_scores_codex":[0.999728,0.00005571189,0.00001877158,0.0000984174,0.00006729558,0.00003169603],"domain_scores_gemma":[0.9995703,0.000174632,0.0000644258,0.00006792038,0.00004285368,0.00007980052],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000009385996,0.00001109311,0.00003124503,0.00001758759,0.00000472402,0.00001109632,0.000007798782,0.00003944675,0.9991795,0.00008142537,0.00001243498,0.0005941587],"study_design_scores_gemma":[0.000005790966,0.00003582104,0.0006500027,0.000003067165,0.00001142746,0.00014201,0.000008491981,0.0008426246,0.9965922,0.0001101577,0.001593174,0.000005163128],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.686862,0.006051029,0.3002889,0.0004908225,0.0001939682,0.0003280593,0.001065364,0.0009296547,0.003790134],"genre_scores_gemma":[0.8436731,0.003611986,0.1444073,0.0001860409,0.00003956049,0.0003057982,0.00302718,0.000257101,0.004491966],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001196265,"threshold_uncertainty_score":0.005021453,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05391168772429403,"score_gpt":0.3502838779571694,"score_spread":0.2963721902328753,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}