{"id":"W2261076239","doi":"10.3791/53301","title":"Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR","year":2016,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Animal Genetics and Reproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; Alberta Livestock and Meat Agency","keywords":"Sexing; Embryo; Biology; Andrology; Primer (cosmetics); Polymerase chain reaction; DNA; Testis determining factor; genomic DNA; Chromosome; Genetics; Y chromosome; Molecular biology; Chemistry; Gene; Medicine","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001528267,0.001006152,0.0009771559,0.001728563,0.0006330202,0.0006767404,0.000998128,0.001117489,0.006153362],"category_scores_gemma":[0.002775221,0.001250636,0.001432022,0.0006859927,0.001014794,0.0005548531,0.0007443268,0.001851698,0.008678057],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002162218,"about_ca_system_score_gemma":0.0007737321,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004673621,"about_ca_topic_score_gemma":0.001613412,"domain_scores_codex":[0.9969419,0.0005571062,0.0003952472,0.001131346,0.0006487248,0.0003256456],"domain_scores_gemma":[0.9975949,0.0007386962,0.0002985448,0.0005730584,0.0006823905,0.000112499],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000749808,0.00004962128,0.000417139,0.0001428352,0.000007452351,0.0001185764,0.0001164085,0.00006585298,0.988135,0.0002610232,0.0003165824,0.01029453],"study_design_scores_gemma":[0.00003525048,0.0007797165,0.008191035,0.00009183144,0.00009838836,0.0007880995,0.0001141986,0.001499729,0.9643993,0.0004767868,0.0234721,0.00005336926],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1043632,0.001685302,0.8716838,0.0002460077,0.0004130704,0.00384546,0.005052533,0.003081668,0.009628872],"genre_scores_gemma":[0.08651988,0.002752902,0.8738163,0.0003263379,0.0001257892,0.003012263,0.01554309,0.001074612,0.01682896],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.006153362,"threshold_uncertainty_score":0.02058506,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01915997827970572,"score_gpt":0.3614545921840478,"score_spread":0.3422946139043421,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}