{"id":"W2261076239","doi":"10.3791/53301","title":"Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR","year":2016,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Animal Genetics and Reproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; Alberta Livestock and Meat Agency","keywords":"Sexing; Embryo; Biology; Andrology; Primer (cosmetics); Polymerase chain reaction; DNA; Testis determining factor; genomic DNA; Chromosome; Genetics; Y chromosome; Molecular biology; Chemistry; Gene; Medicine","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002543115,0.0001199838,0.0002062128,0.00004137595,0.0000375486,0.00001112229,0.0001483304,0.00008396943,0.00005281997],"category_scores_gemma":[0.00009287058,0.00007749804,0.00007053703,0.00003624669,0.00006992173,0.000008951541,0.000104071,0.00004085579,0.000001429735],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001107952,"about_ca_system_score_gemma":0.00003385733,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000003184669,"about_ca_topic_score_gemma":1.589395e-7,"domain_scores_codex":[0.9990565,0.00005767656,0.0003925287,0.0001816135,0.0001741021,0.0001375503],"domain_scores_gemma":[0.9991519,0.000007560821,0.0004057481,0.0002062868,0.0001423828,0.0000861489],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002068496,0.0001026087,0.00049542,0.000008347231,0.000105738,0.00000211762,0.0001009109,0.000001015177,0.9930057,0.00001613126,0.004592284,0.001362855],"study_design_scores_gemma":[0.002046106,0.0007258443,0.0006193414,0.00004949695,0.00002228631,0.0000312344,0.0001269708,0.000004758084,0.9844399,0.00004924185,0.01177944,0.0001053635],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9907497,0.004910913,0.003713489,0.0001408125,0.0002151928,0.00007689147,0.00001065249,0.000002302818,0.0001800662],"genre_scores_gemma":[0.9970793,0.001185551,0.0005504529,0.00005307735,0.0002828604,0.000001863586,0.00000327295,0.00001833868,0.0008252416],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.008565805,"threshold_uncertainty_score":0.3160278,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01915997827970572,"score_gpt":0.3614545921840478,"score_spread":0.3422946139043421,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}