{"id":"W2271093223","doi":"10.1021/acs.biochem.5b01298","title":"Structural and Biochemical Characterization of a Copper-Binding Mutant of the Organomercurial Lyase MerB: Insight into the Key Role of the Active Site Aspartic Acid in Hg–Carbon Bond Cleavage and Metal Binding Specificity","year":2016,"lang":"en","type":"article","venue":"Biochemistry","topic":"Trace Elements in Health","field":"Nursing","cited_by":20,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal","funders":"Division of Chemistry; Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Ministry of Higher Education; Ministry of Higher Education and Scientific Research; U.S. Department of Energy","keywords":"Chemistry; Mutant; Active site; Stereochemistry; Biochemistry; Aspartic acid; Cleavage (geology); Copper; Binding site; Enzyme; Amino acid; Organic chemistry; Biology; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001424427,0.0004376541,0.0003541857,0.0001475287,0.0001534179,0.000300419,0.0003894106,0.0004773146,0.000555261],"category_scores_gemma":[0.0002318838,0.0002447204,0.0002998461,0.0002617101,0.0002378121,0.0001451097,0.0002712692,0.0005991071,0.0003889689],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002860542,"about_ca_system_score_gemma":0.0001746627,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001643796,"about_ca_topic_score_gemma":0.001241634,"domain_scores_codex":[0.9998674,0.0000191889,0.00001605135,0.00002594036,0.00005376233,0.00001759996],"domain_scores_gemma":[0.9997817,0.00003083745,0.00006860888,0.00002455375,0.00002929317,0.00006498522],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007681494,0.00002855601,0.0002782943,0.00001643549,0.000004697146,0.00008855404,0.00001519143,0.00007103848,0.9989622,0.00003762821,0.00001812209,0.0004025694],"study_design_scores_gemma":[0.00002890849,0.0002144523,0.01408344,0.000008002084,0.00001797326,0.001122799,0.00008542861,0.002298873,0.9802701,0.00004670703,0.001807192,0.00001610544],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9978577,0.0002529509,0.001150776,0.00007034974,0.000008503259,0.0000101601,0.000362505,0.0000277315,0.0002593387],"genre_scores_gemma":[0.9940192,0.0002423215,0.00317584,0.00003489454,0.000004978334,0.00001353681,0.001391657,0.00004782595,0.001069959],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001643796,"threshold_uncertainty_score":0.003268421,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009190612191412916,"score_gpt":0.233346357660142,"score_spread":0.2241557454687291,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}