{"id":"W2277915335","doi":"10.1021/acs.biochem.5b01133","title":"Concurrent Increases and Decreases in Local Stability and Conformational Heterogeneity in Cu, Zn Superoxide Dismutase Variants Revealed by Temperature-Dependence of Amide Chemical Shifts","year":2016,"lang":"en","type":"article","venue":"Biochemistry","topic":"Amyotrophic Lateral Sclerosis Research","field":"Medicine","cited_by":40,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hospital for Sick Children; University of Toronto; University of Waterloo","funders":"Canadian Institutes of Health Research","keywords":"Chemistry; Amide; Mutant; Biophysics; Crystallography; Superoxide dismutase; SOD1; Chemical shift; Biochemistry; Physical chemistry; Enzyme; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001384821,0.0002267262,0.0001721241,0.0001850868,0.0001161186,0.00022288,0.000160735,0.000176145,0.0007296911],"category_scores_gemma":[0.0002484759,0.0001565033,0.0001752038,0.0001475515,0.0002615382,0.0001705357,0.0001913758,0.0004874843,0.00015526],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001400724,"about_ca_system_score_gemma":0.00005938072,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003943926,"about_ca_topic_score_gemma":0.0003685025,"domain_scores_codex":[0.9998909,0.00001268827,0.00001102814,0.00003954488,0.00002700558,0.00001864439],"domain_scores_gemma":[0.9997426,0.00003400531,0.0001110359,0.00002961993,0.00003777277,0.00004496533],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007128803,0.000008969581,0.001130948,0.000008482128,0.000008565236,0.00002259432,0.00002957576,0.00003731492,0.998325,0.00001433353,0.000008217969,0.00033454],"study_design_scores_gemma":[0.00001018034,0.0002791971,0.1012288,0.000005044341,0.00003345459,0.0006092851,0.0001015119,0.001450961,0.8957442,0.0000979024,0.0004258653,0.00001371458],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9984194,0.0002209198,0.0009340981,0.0000136362,0.000003599638,0.000004828857,0.000159907,0.00002735799,0.000216256],"genre_scores_gemma":[0.9984994,0.0001311559,0.0007058734,0.00001748465,0.000003116371,0.00001623669,0.0002729986,0.00001501293,0.0003388111],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007296911,"threshold_uncertainty_score":0.002441049,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01556046442108938,"score_gpt":0.2734301650508641,"score_spread":0.2578697006297747,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}