{"id":"W2280466813","doi":"10.1177/1947603515609399","title":"Efficient, Low-Cost Nucleofection of Passaged Chondrocytes","year":2015,"lang":"en","type":"article","venue":"Cartilage","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":18,"is_retracted":false,"has_abstract":true,"ca_institutions":"Mount Sinai Hospital; Lunenfeld-Tanenbaum Research Institute; University of Toronto","funders":"Canadian Arthritis Network; Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; University of Toronto","keywords":"Nucleofection; Transfection; Chondrocyte; Molecular biology; Cell culture; Flow cytometry; Viability assay; Biology; Chemistry; Andrology; Cartilage; Medicine; Anatomy; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001128192,0.00008023738,0.00009169611,0.00002851713,0.00002227977,0.000008643429,0.00009736747,0.00007987053,0.00001639057],"category_scores_gemma":[0.0000812164,0.00007511346,0.00006569263,0.00006070798,0.00004541348,0.000001522062,0.00004747265,0.00004082108,0.00004917873],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001142341,"about_ca_system_score_gemma":0.00004175885,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000007020752,"about_ca_topic_score_gemma":0.00001141722,"domain_scores_codex":[0.999455,0.00003071762,0.0001268747,0.0001681462,0.00008717443,0.0001320603],"domain_scores_gemma":[0.9995313,0.000004382704,0.00005285498,0.0002251333,0.0001142439,0.00007211175],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006910008,0.00003979322,0.00127192,0.000008305922,0.000006993218,0.000002119754,0.00009617321,0.0005132588,0.9921914,0.000007575636,0.005247906,0.0005454471],"study_design_scores_gemma":[0.0003547791,0.0001567033,0.0007034562,0.00001178043,0.000009416319,0.000005308363,0.0001589432,0.0001723089,0.9928595,0.000009017306,0.005469868,0.00008884706],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9952421,0.0002389892,0.0006549239,0.0000176524,0.0002513883,0.00009510204,0.00001055414,0.00001010398,0.003479228],"genre_scores_gemma":[0.9990019,0.00001326144,0.00004344927,0.00008179779,0.0001157147,0.000009283713,0.00003054129,0.000009910755,0.0006941619],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003759821,"threshold_uncertainty_score":0.3063038,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01805045023416465,"score_gpt":0.252079661186395,"score_spread":0.2340292109522304,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}