{"id":"W2280466813","doi":"10.1177/1947603515609399","title":"Efficient, Low-Cost Nucleofection of Passaged Chondrocytes","year":2015,"lang":"en","type":"article","venue":"Cartilage","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":18,"is_retracted":false,"has_abstract":true,"ca_institutions":"Mount Sinai Hospital; Lunenfeld-Tanenbaum Research Institute; University of Toronto","funders":"Canadian Arthritis Network; Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; University of Toronto","keywords":"Nucleofection; Transfection; Chondrocyte; Molecular biology; Cell culture; Flow cytometry; Viability assay; Biology; Chemistry; Andrology; Cartilage; Medicine; Anatomy; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007421019,0.0007301595,0.0007116093,0.0005115852,0.0003736659,0.0006287819,0.0005872026,0.0005418502,0.00183053],"category_scores_gemma":[0.0003740426,0.0002377445,0.0003746936,0.0003222014,0.000267604,0.0002491655,0.0003583784,0.0006233764,0.001353089],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004018508,"about_ca_system_score_gemma":0.00035571,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008501249,"about_ca_topic_score_gemma":0.002826259,"domain_scores_codex":[0.9992582,0.00009753351,0.00008304579,0.0001498714,0.0003445719,0.00006668515],"domain_scores_gemma":[0.99977,0.00005180232,0.00004722182,0.00004701821,0.00005664392,0.00002741878],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002762972,0.00001544039,0.00003264668,0.00006604068,0.000002142442,0.0000380617,0.00001729187,0.00003265683,0.997512,0.0000505878,0.00006337198,0.002142176],"study_design_scores_gemma":[0.000008721202,0.0002455946,0.001205059,0.00002275338,0.00001377349,0.0004341516,0.00001636733,0.0004488364,0.9892212,0.00003187249,0.008340443,0.00001113407],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6505613,0.02294278,0.3035089,0.0002811482,0.000462198,0.002452621,0.00380964,0.001571905,0.01440939],"genre_scores_gemma":[0.7120085,0.01103551,0.2480024,0.0001865082,0.00006977283,0.001563599,0.006522454,0.0004291502,0.02018217],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00183053,"threshold_uncertainty_score":0.006123722,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01805045023416465,"score_gpt":0.252079661186395,"score_spread":0.2340292109522304,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}