{"id":"W2280489664","doi":"10.1021/jacs.5b11814","title":"Understanding Programming of Fungal Iterative Polyketide Synthases: The Biochemical Basis for Regioselectivity by the Methyltransferase Domain in the Lovastatin Megasynthase","year":2015,"lang":"en","type":"article","venue":"Journal of the American Chemical Society","topic":"Microbial Natural Products and Biosynthesis","field":"Medicine","cited_by":95,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"National Center for Chronic Disease Prevention and Health Promotion; National Institute of General Medical Sciences","keywords":"Chemistry; Polyketide; Polyketide synthase; Stereochemistry; Regioselectivity; Acyltransferase; Substrate (aquarium); Enzyme; Moiety; Methyltransferase; Biosynthesis; Cofactor; Combinatorial chemistry; Biochemistry; Methylation; Gene; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001311677,0.0002783824,0.0001829443,0.00009779669,0.0001138389,0.0004337785,0.000312696,0.0003131608,0.000321193],"category_scores_gemma":[0.0001454107,0.0001562695,0.000167853,0.00009154907,0.0002826898,0.0008221544,0.0001577809,0.0004894314,0.0002058971],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003556543,"about_ca_system_score_gemma":0.0002446962,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003379387,"about_ca_topic_score_gemma":0.0006692985,"domain_scores_codex":[0.9999483,0.000006052946,0.000004996275,0.00001729264,0.00001217004,0.00001121207],"domain_scores_gemma":[0.9999541,0.00001111354,0.00001438069,0.000007785855,0.000005456099,0.000007137735],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004612459,0.00001165322,0.0008335434,0.00004156995,0.000002607385,0.00004165436,0.00002855233,0.0009976802,0.9915766,0.001264659,0.00001431824,0.005141082],"study_design_scores_gemma":[0.00000521231,0.0001406601,0.004950399,0.00001047943,0.00001455542,0.0003493904,0.00008426367,0.0105881,0.9788238,0.002117577,0.002903624,0.00001185578],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9667477,0.002985138,0.02796154,0.0002782482,0.00001128482,0.000015753,0.00008648581,0.00009809307,0.001815658],"genre_scores_gemma":[0.9871709,0.002078294,0.01008883,0.00004882023,0.000009173436,0.000009186226,0.00007313138,0.00001289324,0.0005087524],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0004337785,"threshold_uncertainty_score":0.002580523,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05325767633930958,"score_gpt":0.2980436894810587,"score_spread":0.2447860131417492,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}