{"id":"W2284413061","doi":"10.1038/srep19675","title":"Sequence features associated with the cleavage efficiency of CRISPR/Cas9 system","year":2016,"lang":"en","type":"article","venue":"Scientific Reports","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":204,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Core Research for Evolutional Science and Technology; Japan Science and Technology Agency; National Center of Neurology and Psychiatry; Japan Society for the Promotion of Science; Japan Science Society; Takeda Pharmaceutical Company","keywords":"CRISPR; Cas9; Guide RNA; Endonuclease; Computational biology; Genome editing; Cleavage (geology); Biology; DNA; Subgenomic mRNA; Context (archaeology); Genetics; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005419158,0.0004497414,0.0004635029,0.0007152822,0.0001111906,0.0005278584,0.0002331379,0.0003663572,0.000756926],"category_scores_gemma":[0.001956732,0.0002354095,0.0002574253,0.0003929606,0.0001593796,0.0002804924,0.0001664663,0.0005462088,0.0004228183],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003950227,"about_ca_system_score_gemma":0.0002725992,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005810271,"about_ca_topic_score_gemma":0.0005677587,"domain_scores_codex":[0.9988995,0.0002239846,0.00014891,0.0002036561,0.0004207208,0.0001032298],"domain_scores_gemma":[0.9979258,0.0007150809,0.0007114815,0.0001302636,0.0003440147,0.0001733722],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006775634,0.00002664635,0.00269496,0.00005804362,0.00001048464,0.00006070135,0.00001415779,0.0003561022,0.9934523,0.00004854802,0.0000276112,0.003182613],"study_design_scores_gemma":[0.000002038894,0.0002022458,0.03462559,0.0000075333,0.0000300718,0.0005685718,0.00002441006,0.002953306,0.9607883,0.00009615039,0.0006843592,0.00001735241],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9794129,0.001753413,0.01526284,0.00006498872,0.00002149265,0.00005201175,0.0005394381,0.0001399619,0.002753001],"genre_scores_gemma":[0.9916852,0.0005498781,0.006282941,0.00002910539,0.000007644282,0.00002486052,0.0007068498,0.00002754776,0.0006859729],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.000756926,"threshold_uncertainty_score":0.002866149,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006915400733105888,"score_gpt":0.2588820278216165,"score_spread":0.2519666270885106,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}