{"id":"W2295326929","doi":"10.1016/j.stem.2016.01.022","title":"CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs","year":2016,"lang":"en","type":"article","venue":"Cell stem cell","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":549,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Eunice Kennedy Shriver National Institute of Child Health and Human Development; National Institute of Biomedical Imaging and Bioengineering; Howard Hughes Medical Institute; National Institute of Allergy and Infectious Diseases; National Institute of General Medical Sciences; National Institutes of Health; Canadian Institutes of Health Research; Innovative Genomics Institute; University of California, San Francisco; Center for AIDS Research, University of Washington; National Heart, Lung, and Blood Institute; Gladstone Institutes; National Institute on Drug Abuse","keywords":"Biology; CRISPR; CRISPR interference; Induced pluripotent stem cell; Gene silencing; Cas9; Genome editing; RNA interference; Psychological repression; Regulation of gene expression; Gene expression; Gene; Cell biology; Gene knockdown; Genetics; Computational biology; Transcription activator-like effector nuclease; Embryonic stem cell; RNA","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003569793,0.0004067479,0.000467777,0.0003732551,0.0002235709,0.0005824693,0.0003717884,0.0004494737,0.00164792],"category_scores_gemma":[0.0002284956,0.0002803727,0.0005846703,0.0001887816,0.0003106967,0.0001940702,0.0003564199,0.0009275626,0.001160205],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000311044,"about_ca_system_score_gemma":0.000354395,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007034584,"about_ca_topic_score_gemma":0.0009454407,"domain_scores_codex":[0.9996406,0.00003399483,0.00004988756,0.00008411172,0.0001167758,0.00007458981],"domain_scores_gemma":[0.9998118,0.00005385648,0.00003394435,0.0000547405,0.0000218241,0.00002385493],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002692278,0.00001305614,0.0001053093,0.00002093376,0.000008822627,0.00009791875,0.00001769614,0.000131567,0.9972633,0.0003837478,0.0001465408,0.001784302],"study_design_scores_gemma":[0.000005181408,0.00001743342,0.0007240357,0.000002882249,0.00001375111,0.0001665053,0.000007792804,0.0006060621,0.9952917,0.00006814195,0.003093687,0.000002789533],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9008427,0.002169289,0.07079461,0.0005230661,0.0002410313,0.000239943,0.004128318,0.002246194,0.01881492],"genre_scores_gemma":[0.9743986,0.000751389,0.009728543,0.00013582,0.00002392863,0.0001038982,0.002165489,0.0003122419,0.01238002],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00164792,"threshold_uncertainty_score":0.005512834,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01143742308508977,"score_gpt":0.2475092689672364,"score_spread":0.2360718458821466,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}