{"id":"W2301123331","doi":"10.1073/pnas.1519389113","title":"Inhibition of SCF ubiquitin ligases by engineered ubiquitin variants that target the Cul1 binding site on the Skp1–F-box interface","year":2016,"lang":"en","type":"article","venue":"Proceedings of the National Academy of Sciences","topic":"Ubiquitin and proteasome pathways","field":"Biochemistry, Genetics and Molecular Biology","cited_by":74,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institute for Research in Immunology and Cancer; Université de Montréal; Lunenfeld-Tanenbaum Research Institute; Mount Sinai Hospital; University of Toronto","funders":"Basic Energy Sciences; National Center for Research Resources; National Institute of General Medical Sciences; Canadian Cancer Society Research Institute; Canadian Institutes of Health Research; National Institutes of Health; Government of Canada; U.S. Department of Energy","keywords":"Skp1; Ubiquitin; F-box protein; Ubiquitin ligase; Chemistry; Cell biology; Biology; Biochemistry; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001738986,0.0004111101,0.0002525156,0.0001308418,0.00008838885,0.0002658526,0.0002295017,0.0002140193,0.0004460874],"category_scores_gemma":[0.00008336142,0.0001368829,0.0002331616,0.0001315392,0.0001568272,0.0001146405,0.0001348012,0.0003500683,0.0001632899],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004018651,"about_ca_system_score_gemma":0.000142624,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004721491,"about_ca_topic_score_gemma":0.0009083369,"domain_scores_codex":[0.9998645,0.00001957321,0.00001300578,0.00002525773,0.00003992294,0.00003777771],"domain_scores_gemma":[0.9999573,0.000009657801,0.0000132382,0.000004612968,0.000005627036,0.000009596701],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000696679,0.00004336108,0.0001377639,0.00002039321,0.00000911793,0.00002721779,0.000005978738,0.0003028678,0.9978326,0.0001187888,0.0000296438,0.001402432],"study_design_scores_gemma":[0.000008113125,0.00009144858,0.0003062415,0.000001125757,0.00000628169,0.00005248902,0.000001888986,0.0009325522,0.9980559,0.000008569071,0.0005339365,0.000001390743],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.991732,0.0009213422,0.005936164,0.00004052931,0.0000195269,0.00003456152,0.0001257643,0.00006901695,0.001121103],"genre_scores_gemma":[0.993652,0.0005322877,0.004062441,0.0000334877,0.000003137392,0.00002005088,0.0001979723,0.00001398013,0.001484733],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0004721491,"threshold_uncertainty_score":0.0029158,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02944277414350102,"score_gpt":0.2704599035865335,"score_spread":0.2410171294430325,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}