{"id":"W2307605850","doi":"10.1371/journal.pone.0149907","title":"In Situ Staining and Laser Capture Microdissection of Lymph Node Residing SIV Gag-Specific CD8+ T cells—A Tool to Interrogate a Functional Immune Response Ex Vivo","year":2016,"lang":"en","type":"article","venue":"PLoS ONE","topic":"HIV Research and Treatment","field":"Immunology and Microbiology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba; Public Health Agency of Canada","funders":"James B. Pendleton Charitable Trust; Svenska Sällskapet för Medicinsk Forskning; Svenska Läkaresällskapet; National Institutes of Health; Vetenskapsrådet; National Institute of Allergy and Infectious Diseases; Bill and Melinda Gates Foundation","keywords":"Laser capture microdissection; Ex vivo; Biology; CD8; Cytotoxic T cell; Immune system; Lymph node; Molecular biology; T cell; Immunology; In vivo; In vitro; Gene expression; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003120923,0.0003719128,0.0002180607,0.0004023613,0.0004649123,0.0004022829,0.0002544432,0.0003321632,0.001768084],"category_scores_gemma":[0.0001599812,0.0002130882,0.0002567334,0.0003122453,0.0003972868,0.0002019882,0.0002588318,0.0006878931,0.0005439076],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000220657,"about_ca_system_score_gemma":0.0002664733,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005463858,"about_ca_topic_score_gemma":0.00181358,"domain_scores_codex":[0.9997193,0.00003651842,0.00001885795,0.000107067,0.00006138707,0.00005686816],"domain_scores_gemma":[0.9998388,0.00005037626,0.00003452076,0.00003389836,0.00002258889,0.00001968059],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001619819,0.000006216047,0.00009822317,0.00001382132,0.000001372613,0.00001088197,0.0000185547,0.00001696551,0.9991881,0.00003535933,0.00001412616,0.0005801112],"study_design_scores_gemma":[0.000006363429,0.0001738326,0.004420353,0.000003753818,0.00000951839,0.0001634329,0.00006211513,0.0006838972,0.9925243,0.0001063849,0.0018414,0.000004637168],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8398759,0.001266053,0.1516628,0.0002449527,0.0001534663,0.0003502187,0.001676569,0.0004361173,0.004333698],"genre_scores_gemma":[0.8064879,0.001885894,0.1756566,0.0003963585,0.0001140067,0.001024393,0.001649668,0.0002109757,0.01257413],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001768084,"threshold_uncertainty_score":0.005914807,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02638477098916893,"score_gpt":0.2294697859882083,"score_spread":0.2030850149990393,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}