{"id":"W2312210365","doi":"10.1021/bc200672s","title":"Site-Specific Fluorescent Labeling and Oriented Immobilization of a Triple Mutant of CYP3A4 via C64","year":2012,"lang":"en","type":"article","venue":"Bioconjugate Chemistry","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":17,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; McGill University","keywords":"Chemistry; Maleimide; Enzyme; Cysteine; Mutant; Biochemistry; CYP3A4; Steric effects; Stereochemistry; Cytochrome P450; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000188234,0.0001455679,0.0001981795,0.00002376274,0.00003459207,0.000006179036,0.00007974191,0.000156358,0.00004982436],"category_scores_gemma":[0.00006482942,0.0001394921,0.00007310005,0.0001377564,0.0001106095,0.00000744032,0.00008198548,0.00004607834,0.00000173759],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001394904,"about_ca_system_score_gemma":0.00002320262,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000005819913,"about_ca_topic_score_gemma":6.1514e-7,"domain_scores_codex":[0.9990523,0.00002105775,0.0003702745,0.0002471377,0.0001187393,0.0001904808],"domain_scores_gemma":[0.9992083,0.000006851442,0.0002172725,0.0003136411,0.0001681724,0.00008580426],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007035975,0.0001415624,0.00425624,0.0001682292,0.00002919413,1.712185e-7,0.00008459424,0.000008015715,0.9947202,0.0000323373,0.0001459847,0.0003431017],"study_design_scores_gemma":[0.0005520129,0.00005141013,0.0004419344,0.00003505617,0.00002923166,0.000006800251,0.0001018585,0.00008697178,0.9929236,0.000005688831,0.005623859,0.0001415784],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9938434,0.004416177,0.001238264,0.000006702981,0.00006711438,0.0001562385,0.00004820201,0.000007824691,0.0002160938],"genre_scores_gemma":[0.9978428,0.0007857652,0.0003463624,0.00001281769,0.0001207772,0.00001243244,0.0007191385,0.00001969342,0.0001401855],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.005477874,"threshold_uncertainty_score":0.5688321,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008838134466399637,"score_gpt":0.22419426700071,"score_spread":0.2153561325343104,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}