{"id":"W2312210365","doi":"10.1021/bc200672s","title":"Site-Specific Fluorescent Labeling and Oriented Immobilization of a Triple Mutant of CYP3A4 via C64","year":2012,"lang":"en","type":"article","venue":"Bioconjugate Chemistry","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":17,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; McGill University","keywords":"Chemistry; Maleimide; Enzyme; Cysteine; Mutant; Biochemistry; CYP3A4; Steric effects; Stereochemistry; Cytochrome P450; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001490302,0.0003567942,0.0002088502,0.0001770989,0.0001538378,0.0001677199,0.0002603323,0.0003922094,0.0005917992],"category_scores_gemma":[0.0001054803,0.0001642224,0.0002889253,0.0001583839,0.0002135648,0.000115795,0.0002064615,0.0004091675,0.0003374648],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003135713,"about_ca_system_score_gemma":0.0001511361,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007236321,"about_ca_topic_score_gemma":0.0008002883,"domain_scores_codex":[0.9998534,0.00001852936,0.00001370516,0.00003862387,0.00004816065,0.00002757398],"domain_scores_gemma":[0.999916,0.000009395171,0.00003197828,0.00001504415,0.00001242848,0.0000153063],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001076724,0.000003644068,0.00002411272,0.000004993578,0.000001458722,0.00001423648,0.000002948662,0.00002632835,0.9996887,0.00002300878,0.000006072592,0.0001937542],"study_design_scores_gemma":[0.000002011191,0.00003510529,0.0002272157,4.377394e-7,0.000002702232,0.00006105405,0.000002078939,0.0002117455,0.9991418,0.00000577498,0.0003082199,0.000001717864],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9897128,0.0002827006,0.0087259,0.00006313824,0.00001905078,0.00002889893,0.0001623116,0.00009369964,0.0009115734],"genre_scores_gemma":[0.987209,0.0003095621,0.01011674,0.00002665109,0.000004604871,0.00003928299,0.0002916744,0.00003010013,0.001972496],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007236321,"threshold_uncertainty_score":0.002275109,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008838134466399637,"score_gpt":0.22419426700071,"score_spread":0.2153561325343104,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}