{"id":"W2312534110","doi":"10.1016/j.bpj.2014.11.2978","title":"Biophysical Analysis of a Successful Protocol to Reconstitute Tetramers of the M2 Muscarinic Receptor","year":2015,"lang":"en","type":"article","venue":"Biophysical Journal","topic":"Lipid Membrane Structure and Behavior","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto","funders":"","keywords":"Isothermal titration calorimetry; Chemistry; Sodium Cholate; Micelle; Lipid bilayer; Vesicle; Biophysics; Phosphatidylcholine; Bilayer; Fluorescence anisotropy; Chromatography; Digitonin; Differential scanning calorimetry; Membrane; Biochemistry; Organic chemistry; Phospholipid","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007537112,0.0006429204,0.0004820911,0.0002458683,0.0006305562,0.0004862857,0.0007488432,0.0007161126,0.001816785],"category_scores_gemma":[0.001293306,0.0002858505,0.0004849468,0.0004533892,0.0003430469,0.0003473273,0.0004014294,0.001796272,0.001008762],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006967766,"about_ca_system_score_gemma":0.0005435297,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001927927,"about_ca_topic_score_gemma":0.003219155,"domain_scores_codex":[0.9993531,0.000117287,0.00005844325,0.0001075795,0.0002372762,0.0001262183],"domain_scores_gemma":[0.9995259,0.000149471,0.00004244797,0.0001177183,0.0001230844,0.00004144687],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004568278,0.0000798782,0.00007372794,0.00002542217,0.00001812184,0.00006091638,0.00005664683,0.0001739008,0.9975859,0.0002228641,0.0003011101,0.001355924],"study_design_scores_gemma":[0.00001747897,0.00008725309,0.0005705222,0.000002705026,0.00001716213,0.00006950144,0.00002025845,0.001420689,0.9952462,0.00007842462,0.002458597,0.00001119024],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.9233171,0.0009657633,0.06708273,0.001289426,0.0003667444,0.0008580527,0.001674589,0.0005048387,0.00394078],"genre_scores_gemma":[0.9190499,0.001828657,0.05800563,0.0007198307,0.0001090891,0.001287307,0.004119129,0.0005012248,0.01437922],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001927927,"threshold_uncertainty_score":0.006077707,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01828678185229218,"score_gpt":0.2980882449234375,"score_spread":0.2798014630711453,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}