{"id":"W2312970880","doi":"10.1097/pai.0000000000000202","title":"Ki-67 Membranous Staining: Biologically Relevant or an Artifact of Multiplexed Immunofluorescent Staining","year":2015,"lang":"en","type":"article","venue":"Applied immunohistochemistry & molecular morphology","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; Health Sciences Centre; Sunnybrook Health Science Centre","funders":"","keywords":"Staining; Cyanine; Polyclonal antibodies; Molecular biology; Antibody; Immunohistochemistry; Primary and secondary antibodies; Chemistry; Multiplex; Cytokeratin; Pathology; Biology; Medicine; Immunology; Fluorescence","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0006536901,0.0006052835,0.0006765143,0.0001016361,0.0001573941,0.0000276408,0.0009193352,0.0008350932,0.00007515528],"category_scores_gemma":[0.0003253305,0.0005515565,0.0002516835,0.0002695804,0.0007580492,0.000008017048,0.0005405438,0.0004387258,0.00001840096],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001182081,"about_ca_system_score_gemma":0.0004880235,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00008651141,"about_ca_topic_score_gemma":8.465537e-7,"domain_scores_codex":[0.99652,0.000188591,0.0009637407,0.001235216,0.0002925983,0.0007998449],"domain_scores_gemma":[0.9967505,0.00003424155,0.0005518335,0.001938666,0.000390531,0.0003342127],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001457928,0.0004087373,0.00008515706,0.0000253043,0.0001772056,0.00009260944,0.0001059282,0.0002185515,0.9945843,0.0007654075,0.0005646698,0.001514208],"study_design_scores_gemma":[0.00178167,0.001311034,0.0001321312,0.00001483691,0.00007515276,0.0003176763,0.0004425712,0.00005298812,0.9899774,0.0004303918,0.004805526,0.0006586357],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9830962,0.001479434,0.01185707,0.0001456712,0.0001026892,0.0007837231,0.0000670368,0.0001358152,0.002332396],"genre_scores_gemma":[0.9763396,0.0001462329,0.02049211,0.0006537596,0.00007901883,0.0004684762,0.00144944,0.0001017474,0.0002696012],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.008635038,"threshold_uncertainty_score":0.9996936,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0279739838426252,"score_gpt":0.3061930509059133,"score_spread":0.2782190670632881,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}