{"id":"W2313674900","doi":"10.1021/sb200007r","title":"Parts-Based Assembly of Synthetic Transmembrane Proteins in Mammalian Cells","year":2011,"lang":"en","type":"letter","venue":"ACS Synthetic Biology","topic":"Cellular transport and secretion","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Canadian Institutes of Health Research","keywords":"Endoplasmic reticulum; Transmembrane protein; Golgi apparatus; Transmembrane domain; Cytoplasm; Membrane protein; Cell biology; Fusion protein; Sec61; Signal peptide; STIM1; Biology; Integral membrane protein; Membrane; Biochemistry; Peptide sequence; Translocon; Recombinant DNA; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003580736,0.000420134,0.000238243,0.0001843472,0.0002108774,0.0003925812,0.0003013357,0.0009596166,0.0005959623],"category_scores_gemma":[0.0005738264,0.000276556,0.0002770165,0.0001947632,0.0004331905,0.0003203206,0.0004307084,0.0007139692,0.00121043],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005669138,"about_ca_system_score_gemma":0.000117631,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001884947,"about_ca_topic_score_gemma":0.000190867,"domain_scores_codex":[0.9995407,0.0001631379,0.00003708412,0.00005656929,0.0001764101,0.00002609781],"domain_scores_gemma":[0.9998118,0.00006149095,0.00003798775,0.00004349688,0.00002923595,0.00001600326],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006263488,0.00001307834,0.00009911512,0.0001052667,0.000009534221,0.0002806573,0.00006104034,0.001169182,0.9875491,0.004316311,0.00112351,0.005210614],"study_design_scores_gemma":[0.00004546021,0.0003136889,0.0003608195,0.00001657971,0.0000128165,0.0008559603,0.00002980387,0.008296774,0.9401259,0.001479396,0.04844006,0.00002276797],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7164502,0.009328732,0.2261669,0.006433195,0.002113587,0.0004480729,0.001053477,0.002054029,0.03595176],"genre_scores_gemma":[0.8776029,0.0042714,0.1006034,0.001627789,0.0001430997,0.0003338843,0.001214993,0.0002598573,0.01394257],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009596166,"threshold_uncertainty_score":0,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0150889294209669,"score_gpt":0.2244392353163119,"score_spread":0.209350305895345,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}