{"id":"W2313686433","doi":"10.1139/w11-073","title":"Gene cloning, structural gene and promoter identification, and active assay of the phosphatidylcholine synthase of <i>Pseudomonas</i> sp. strain 593","year":2011,"lang":"en","type":"article","venue":"Canadian Journal of Microbiology","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Science Foundation","keywords":"Open reading frame; ORFS; Plasmid; Biology; Molecular biology; Shuttle vector; Molecular cloning; Promoter; Gene; Escherichia coli; Cloning (programming); XhoI; Expression vector; Complementary DNA; Gene expression; Genetics; Peptide sequence; Vector (molecular biology); Recombinant DNA; EcoRI","routes":{"ca_aff":false,"ca_fund":false,"ca_venue":true,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001724574,0.0005750009,0.0004757405,0.0003982518,0.000227919,0.0003272076,0.0003367816,0.0003364718,0.0009920633],"category_scores_gemma":[0.000347395,0.0002298098,0.000571228,0.0006538376,0.0003409689,0.0002045266,0.0003242111,0.0007400926,0.001183534],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005579061,"about_ca_system_score_gemma":0.0004499809,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003098909,"about_ca_topic_score_gemma":0.00206124,"domain_scores_codex":[0.9996998,0.00003149525,0.00002583948,0.00005540984,0.0001375047,0.00004996427],"domain_scores_gemma":[0.9997721,0.00005412688,0.00005269317,0.00002726731,0.00003916814,0.0000546519],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002909918,0.00001714824,0.00009941158,0.00002043798,0.000001015269,0.00002082989,0.000009204628,0.00002005384,0.9992403,0.00003297058,0.00001024343,0.0004992862],"study_design_scores_gemma":[0.0000115001,0.00009733251,0.003794583,0.000005865335,0.00001084389,0.0003279764,0.00004048266,0.0006043283,0.9932033,0.00005402991,0.001842264,0.00000741694],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9687533,0.0008063074,0.02287442,0.0003068192,0.00005088531,0.0002098676,0.004157202,0.0002303031,0.002610817],"genre_scores_gemma":[0.9398921,0.001197314,0.03731332,0.00008849092,0.00003684139,0.0001909531,0.01483723,0.0001963264,0.006247502],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003098909,"threshold_uncertainty_score":0.006161749,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007919351120019043,"score_gpt":0.1987639317995252,"score_spread":0.1908445806795062,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}