{"id":"W2315924005","doi":"10.1021/bi401166f","title":"Kinetic and Structural Evaluation of Selected Active Site Mutants of the <i>Aspergillus fumigatus</i> KDNase (Sialidase)","year":2013,"lang":"en","type":"article","venue":"Biochemistry","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"Simon Fraser University","funders":"Wellcome Trust","keywords":"Aspergillus fumigatus; Active site; Chemistry; Sialidase; Stereochemistry; Nucleophile; Mutagenesis; Mutant; Substrate (aquarium); Hydrolase; Binding site; Biochemistry; Enzyme; Catalysis; Biology; Neuraminidase; Microbiology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002317012,0.0004129181,0.0002816493,0.00009644834,0.0001367648,0.000303615,0.0003565134,0.000287111,0.0005128442],"category_scores_gemma":[0.0004117225,0.0001495635,0.0002982987,0.0001961436,0.0001572199,0.0002047182,0.0001516106,0.0003722746,0.0002629333],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003980897,"about_ca_system_score_gemma":0.0002007259,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002397869,"about_ca_topic_score_gemma":0.001743921,"domain_scores_codex":[0.9997743,0.00003906485,0.00002885749,0.00003976417,0.00008601783,0.00003199696],"domain_scores_gemma":[0.9997936,0.00004937889,0.00005900643,0.00002054286,0.00004485293,0.00003268779],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001497117,0.00006779932,0.0006422678,0.00002956565,0.00001269027,0.00004541126,0.00002171691,0.0005719409,0.9968899,0.00007795196,0.00002873554,0.001462297],"study_design_scores_gemma":[0.00000716124,0.000199198,0.001351198,0.000002274187,0.00001398696,0.0001356354,0.00002174249,0.001882128,0.9958736,0.00001385058,0.0004943967,0.00000488024],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9976141,0.0003806283,0.001402938,0.00002888857,0.000009824785,0.00001234643,0.0001826936,0.00002651847,0.0003419892],"genre_scores_gemma":[0.9964613,0.0002441464,0.002356259,0.00001206955,0.000002244363,0.00001145738,0.0004065693,0.0000251602,0.0004807764],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002397869,"threshold_uncertainty_score":0.004767895,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009085677170828633,"score_gpt":0.2612838731879594,"score_spread":0.2521981960171308,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}