{"id":"W2317605508","doi":"10.1021/bi3016795","title":"Oxidation of Methionine 216 in Sheep and Elk Prion Protein Is Highly Dependent upon the Amino Acid at Position 218 but Is Not Important for Prion Propagation","year":2013,"lang":"en","type":"article","venue":"Biochemistry","topic":"Prion Diseases and Protein Misfolding","field":"Biochemistry, Genetics and Molecular Biology","cited_by":22,"is_retracted":false,"has_abstract":true,"ca_institutions":"Canadian Food Inspection Agency","funders":"","keywords":"Scrapie; Methionine; Prion protein; Western blot; Amino acid; Chronic wasting disease; Amino acid residue; Recombinant DNA; Residue (chemistry); Chemistry; PRNP; Biochemistry; Blot; Biology; Molecular biology; Peptide sequence; Pathology; Medicine; Disease; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002839619,0.0001774021,0.00014316,0.00003110256,0.0001024304,0.00003681659,0.0001383324,0.0002339308,0.00004201502],"category_scores_gemma":[0.0001076025,0.0001451691,0.00007141291,0.00006772101,0.00004883816,0.0000157361,0.0001192965,0.00007302229,0.000002474407],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006246103,"about_ca_system_score_gemma":0.00007157936,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005845508,"about_ca_topic_score_gemma":0.000008344853,"domain_scores_codex":[0.9987682,0.00003966865,0.0003850564,0.0004121888,0.0001950928,0.0001998506],"domain_scores_gemma":[0.9991549,0.00001111949,0.0002908479,0.0003241604,0.0001507948,0.00006812291],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002440481,0.0000583119,0.0005828494,0.0001999516,0.00002337901,4.333419e-7,0.00004904713,0.00000102639,0.9875348,0.00001531211,0.0001834761,0.01110732],"study_design_scores_gemma":[0.0007970624,0.0001670926,0.002184198,0.00007965585,0.00002689417,0.000008239655,0.000052119,0.0004835784,0.9956201,0.0001229212,0.000279895,0.0001782835],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9933184,0.003179418,0.0007530383,0.001337899,0.00002835928,0.001291273,0.00006661472,0.000009582606,0.00001542558],"genre_scores_gemma":[0.9970088,0.0006469502,0.001045796,0.0002097173,0.0001019328,0.0003840126,0.0003563543,0.00001893351,0.0002275282],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01092904,"threshold_uncertainty_score":0.5919825,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007605377168487349,"score_gpt":0.2339650559332513,"score_spread":0.2263596787647639,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}