{"id":"W2317888720","doi":"10.1002/dvdy.24051","title":"A <i>piggyBac</i> transposon‐ and gateway‐enhanced system for efficient BAC transgenesis","year":2014,"lang":"en","type":"article","venue":"Developmental Dynamics","topic":"Animal Genetics and Reproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Institute of Genetics; Institute of Zoology, Chinese Academy of Sciences; RIKEN; Feinberg School of Medicine; University of California, San Francisco; University of California, Santa Barbara; National Institutes of Health; Tokyo Metropolitan University; Technion-Israel Institute of Technology; University of Tokushima; Genome Institute of Singapore; Wakayama Medical University; Chinese Academy of Sciences; University of Queensland; Monash University; National Cancer Institute; University of Bristol; School of Medicine; Newcastle University; University of Dundee; University of South Florida; University of Oxford; Brigham Young University; University College London; Cancer Research UK; Wellcome Trust; National Tsing Hua University; Montana State University; University of Illinois at Urbana-Champaign; University of Dayton; Massachusetts General Hospital; University of Minnesota; Case Western Reserve University; University of Arizona; Northwestern University; University of Miami; Stowers Institute for Medical Research; University of South Carolina; Cincinnati Children's Hospital Medical Center; Brown University; Harvard University; University of Washington; University of Nebraska Medical Center; University of Toronto; Yale University","keywords":"Transgenesis; Biology; Recombineering; Bacterial artificial chromosome; mCherry; Transgene; Genetics; Reporter gene; Gene; Transposase; Transposable element; Green fluorescent protein; Molecular biology; Cell biology; Computational biology; Homologous recombination; Gene expression; Genome; Reproductive technology","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001236564,0.001488482,0.0009324991,0.001625633,0.0006287384,0.0008941192,0.001474756,0.001505835,0.006606164],"category_scores_gemma":[0.0007041952,0.0008245328,0.0009485472,0.0007915251,0.0007729436,0.0008413162,0.001140623,0.002318025,0.01322079],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005149745,"about_ca_system_score_gemma":0.0007066783,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00049405,"about_ca_topic_score_gemma":0.0005767912,"domain_scores_codex":[0.9987277,0.000261374,0.0001760921,0.0003163512,0.000378561,0.000139808],"domain_scores_gemma":[0.9994732,0.00009631052,0.0001535512,0.0001428554,0.00005391227,0.00008029291],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006838564,0.00003799115,0.0000859416,0.0001661845,0.00001139992,0.0001228208,0.00002346772,0.0000575029,0.9901674,0.0007505316,0.0009882003,0.007520184],"study_design_scores_gemma":[0.00005227955,0.0002814441,0.002032972,0.00005315856,0.00008500104,0.002304605,0.00001588647,0.002062459,0.9185907,0.0004119551,0.07405583,0.0000537639],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1413441,0.007198574,0.7921889,0.001235759,0.001548718,0.002903417,0.005459072,0.02809917,0.02002231],"genre_scores_gemma":[0.3205332,0.008124985,0.5913416,0.001048077,0.0003740677,0.003328941,0.02283787,0.003684398,0.04872682],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.006606164,"threshold_uncertainty_score":0.02209979,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003989798768468915,"score_gpt":0.1897896157812468,"score_spread":0.1857998170127779,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}