{"id":"W2317888720","doi":"10.1002/dvdy.24051","title":"A <i>piggyBac</i> transposon‐ and gateway‐enhanced system for efficient BAC transgenesis","year":2014,"lang":"en","type":"article","venue":"Developmental Dynamics","topic":"Animal Genetics and Reproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Institute of Genetics; Institute of Zoology, Chinese Academy of Sciences; RIKEN; Feinberg School of Medicine; University of California, San Francisco; University of California, Santa Barbara; National Institutes of Health; Tokyo Metropolitan University; Technion-Israel Institute of Technology; University of Tokushima; Genome Institute of Singapore; Wakayama Medical University; Chinese Academy of Sciences; University of Queensland; Monash University; National Cancer Institute; University of Bristol; School of Medicine; Newcastle University; University of Dundee; University of South Florida; University of Oxford; Brigham Young University; University College London; Cancer Research UK; Wellcome Trust; National Tsing Hua University; Montana State University; University of Illinois at Urbana-Champaign; University of Dayton; Massachusetts General Hospital; University of Minnesota; Case Western Reserve University; University of Arizona; Northwestern University; University of Miami; Stowers Institute for Medical Research; University of South Carolina; Cincinnati Children's Hospital Medical Center; Brown University; Harvard University; University of Washington; University of Nebraska Medical Center; University of Toronto; Yale University","keywords":"Transgenesis; Biology; Recombineering; Bacterial artificial chromosome; mCherry; Transgene; Genetics; Reporter gene; Gene; Transposase; Transposable element; Green fluorescent protein; Molecular biology; Cell biology; Computational biology; Homologous recombination; Gene expression; Genome; Reproductive technology","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001642296,0.0001590212,0.0001381841,0.00002798009,0.000139452,0.00001911743,0.00008506374,0.0001140317,0.00000141332],"category_scores_gemma":[0.000008870978,0.0001506223,0.00006229488,0.00005756128,0.0000552701,0.000001499319,0.00002409661,0.00003307576,0.000003051145],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003856025,"about_ca_system_score_gemma":0.00003656857,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000047847,"about_ca_topic_score_gemma":0.00003947385,"domain_scores_codex":[0.9990622,0.00002060583,0.0001927666,0.0004250774,0.00008661916,0.0002127297],"domain_scores_gemma":[0.9997014,0.000006227648,0.00004475133,0.0001205833,0.00005365442,0.00007341384],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001882293,0.00004891076,0.0004139831,0.0001860906,0.00005462456,3.180059e-7,0.000144314,0.000586464,0.9797314,0.0006049876,0.00003885057,0.01800181],"study_design_scores_gemma":[0.001993346,0.0006206597,0.007769155,0.00006648843,0.0000751168,0.00007189396,0.0008249906,0.02357029,0.9470417,0.00003085668,0.01718234,0.0007531095],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8376463,0.00012963,0.1609787,0.00005579843,0.0002020383,0.0002955141,0.0000393826,0.00002004363,0.0006325969],"genre_scores_gemma":[0.9914576,0.00007041865,0.007536686,0.00005152145,0.00009949393,0.00005547486,0.0002317242,0.00003023981,0.0004667941],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1538114,"threshold_uncertainty_score":0.6142198,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003989798768468915,"score_gpt":0.1897896157812468,"score_spread":0.1857998170127779,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}