{"id":"W2319382903","doi":"10.1139/w2012-017","title":"Optimized expression of dual reporter genes in transient transfection of purified<i>Toxoplasma gondii</i>using different promoters","year":2012,"lang":"en","type":"article","venue":"Canadian Journal of Microbiology","topic":"Toxoplasma gondii Research Studies","field":"Immunology and Microbiology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Canadian Food Inspection Agency; Agriculture and Agri-Food Canada; University of Lethbridge","funders":"Changjiang Scholar Program of Chinese Ministry of Education; University of South China; Forskningsrådet om Hälsa, Arbetsliv och Välfärd; South China Agricultural University; National Natural Science Foundation of China","keywords":"Reporter gene; Green fluorescent protein; Promoter; Biology; Bioreporter; Transfection; Molecular biology; Luciferase; Gene; Toxoplasma gondii; Fluorescence; Yellow fluorescent protein; Gene expression; Genetics; Antibody","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":true,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007014541,0.0008196442,0.0008165999,0.0003469596,0.0002740371,0.0006609172,0.0006108903,0.0005875751,0.001212163],"category_scores_gemma":[0.0003508735,0.0004878539,0.0006294327,0.0004946122,0.0005089767,0.0003859773,0.0005608773,0.001411003,0.00086115],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005918806,"about_ca_system_score_gemma":0.0003998957,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006661031,"about_ca_topic_score_gemma":0.001208223,"domain_scores_codex":[0.9993278,0.0001251541,0.0000975577,0.0001426216,0.0001787605,0.0001280622],"domain_scores_gemma":[0.9997188,0.00007632707,0.00006465376,0.00004041294,0.00006086386,0.00003877182],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004710157,0.00001888399,0.00005635035,0.00004083842,0.00000260906,0.00001999189,0.0000153389,0.00005397986,0.9989945,0.0000925959,0.00003765399,0.000620135],"study_design_scores_gemma":[0.000007826563,0.00005426984,0.0003103469,0.000004206849,0.000008812961,0.00008125933,0.00001139105,0.0005919776,0.9974445,0.00002150886,0.001458766,0.000005204127],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8223338,0.001495444,0.166901,0.0003072288,0.0002266888,0.0007171587,0.003178535,0.0009483293,0.003891862],"genre_scores_gemma":[0.7491894,0.001895874,0.224421,0.0001706309,0.00003025925,0.001172586,0.008967089,0.0006907317,0.01346235],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001212163,"threshold_uncertainty_score":0.004294395,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02305444362809963,"score_gpt":0.246106774538694,"score_spread":0.2230523309105944,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}